BRENDA - Enzyme Database

Engineering the active site of ascorbate peroxidase

Lloyd Raven, E.; Celik, A.; Cullis, P.M.; Sangar, R.; Sutcliffe, M.J.; Biochem. Soc. Trans. 29, 105-111 (2001) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.11.1.11
recombinant enzyme
Pisum sativum
Engineering
EC Number
Protein Variants
Commentary
Organism
1.11.1.11
W41A
the mutation enables the efficient conversion of recombinant enzyme into a stereoselective oxidizing agent for sulfides
Pisum sativum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.11
cytosol
-
Pisum sativum
5829
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.11.1.11
Pisum sativum
-
pea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.11.1.11
ethyl phenyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
-
-
?
1.11.1.11
isopropyl phenyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
L-ascorbate + H2O2
-
439877
Pisum sativum
dehydroascorbate + H2O
-
439877
Pisum sativum
?
1.11.1.11
methyl naphthalene sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
methyl phenyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
additional information
experimental and modelled enantiomeric ratios R: S for oxidation of thioethers by recombinant enzyme and mutant Trp-41-Ala
439877
Pisum sativum
?
-
-
-
?
1.11.1.11
n-propyl phenyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
p-chlorophenyl methyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
p-cresol + cumene-hydroperoxide
-
439877
Pisum sativum
4a,9b-dihydro-8,9b-dimethyl-3(4H)-dibenzofuranone + 2,2'-dihydroxy-5,5'-dimethylbiphenyl + 1,1-dimethylbenzylalcohol + bis-(1-methyl-1-phenylethyl)peroxide
the formation of bis-(1-methyl-1-phenylethyl)peroxide derives from the reaction of 1,1-dimethylbenzylalcohol with either p-cresol or 2,2'-dihydroxy-5,5'-dimethylbiphenyl
439877
Pisum sativum
?
1.11.1.11
p-cresol + H2O2
-
439877
Pisum sativum
4a,9b-dihydro-8,9b-dimethyl-3(4H)-dibenzofuranone + 2,2'-dihydroxy-5,5'-dimethylbiphenyl + H2O
these products, which are derived from reactions of the p-methylphenoxy radical, itself form as a direct result of single-electron oxidation of p-cresol by the enzyme, can be accommodated from the known chemistry of the radical products, the product ratio 4alpha,9beta-dihydro-8,9beta-dimethyl-3(4H)-dibenzofuranone: 2,2'-dihydroxy-5,5'-dimethylbiphenyl is found to depend on enzyme concentration
439877
Pisum sativum
?
1.11.1.11
p-nitrophenyl methyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.11
recombinant enzyme
Pisum sativum
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.11.1.11
W41A
the mutation enables the efficient conversion of recombinant enzyme into a stereoselective oxidizing agent for sulfides
Pisum sativum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.11
cytosol
-
Pisum sativum
5829
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.11.1.11
ethyl phenyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
-
-
?
1.11.1.11
isopropyl phenyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
L-ascorbate + H2O2
-
439877
Pisum sativum
dehydroascorbate + H2O
-
439877
Pisum sativum
?
1.11.1.11
methyl naphthalene sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
methyl phenyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
additional information
experimental and modelled enantiomeric ratios R: S for oxidation of thioethers by recombinant enzyme and mutant Trp-41-Ala
439877
Pisum sativum
?
-
-
-
?
1.11.1.11
n-propyl phenyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
p-chlorophenyl methyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?
1.11.1.11
p-cresol + cumene-hydroperoxide
-
439877
Pisum sativum
4a,9b-dihydro-8,9b-dimethyl-3(4H)-dibenzofuranone + 2,2'-dihydroxy-5,5'-dimethylbiphenyl + 1,1-dimethylbenzylalcohol + bis-(1-methyl-1-phenylethyl)peroxide
the formation of bis-(1-methyl-1-phenylethyl)peroxide derives from the reaction of 1,1-dimethylbenzylalcohol with either p-cresol or 2,2'-dihydroxy-5,5'-dimethylbiphenyl
439877
Pisum sativum
?
1.11.1.11
p-cresol + H2O2
-
439877
Pisum sativum
4a,9b-dihydro-8,9b-dimethyl-3(4H)-dibenzofuranone + 2,2'-dihydroxy-5,5'-dimethylbiphenyl + H2O
these products, which are derived from reactions of the p-methylphenoxy radical, itself form as a direct result of single-electron oxidation of p-cresol by the enzyme, can be accommodated from the known chemistry of the radical products, the product ratio 4alpha,9beta-dihydro-8,9beta-dimethyl-3(4H)-dibenzofuranone: 2,2'-dihydroxy-5,5'-dimethylbiphenyl is found to depend on enzyme concentration
439877
Pisum sativum
?
1.11.1.11
p-nitrophenyl methyl sulfide + H2O2
-
439877
Pisum sativum
? + H2O
-
439877
Pisum sativum
?