EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.11 | expression in Escherichia coli, most of the enzyme produced is present in the apo-form, without heme | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.11 | 0.02 | - |
H2O2 | recombinant enzyme | Glycine max | |
1.11.1.11 | 0.35 | - |
ascorbate | recombinant enzyme | Glycine max | |
1.11.1.11 | 1.8 | - |
guaiacol | recombinant enzyme | Glycine max | |
1.11.1.11 | 2.4 | - |
pyrogallol | recombinant enzyme | Glycine max |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.11 | cytosol | - |
Glycine max | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.11 | 30000 | - |
x * 30000, SDS-PAGE | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.11 | L-ascorbate + H2O2 | Glycine max | physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation | dehydroascorbate + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.11 | Glycine max | - |
soybean | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.11 | using Ni2+-agarose affinity chromatography and gel filtration after reconstitution with hemin | Glycine max |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.11.1.11 | the apo-recombinant protein is easily converted to the holo-recombinant form by in vitro reconstitution with hemin | Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.11.1.11 | root | nodules | Glycine max | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.11 | additional information | - |
effects of media amendments | Glycine max |
1.11.1.11 | 3 | 4 | affinity purified preparation | Glycine max |
1.11.1.11 | 31.7 | - |
purified recombinant enzyme | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.11 | 2,2'-azino-di-(3-ethyl-benzothiazoline-(6)-sulfonic acid) + H2O2 | 3% relative activity to L-ascorbate | Glycine max | ? + H2O | - |
? | |
1.11.1.11 | guaiacol + H2O2 | 30.5% activity relative to L-ascorbate | Glycine max | ? | - |
? | |
1.11.1.11 | L-ascorbate + H2O2 | - |
Glycine max | dehydroascorbate + H2O | - |
? | |
1.11.1.11 | L-ascorbate + H2O2 | physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation | Glycine max | dehydroascorbate + 2 H2O | - |
? | |
1.11.1.11 | pyrogallol + H2O2 | 723% activity relative to L-ascorbate | Glycine max | 3-hydroxybenzo-1,2-quinone + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.11 | ? | x * 30000, SDS-PAGE | Glycine max |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.11 | 5.9 | 7 | - |
Glycine max |
1.11.1.11 | 7 | - |
assay at | Glycine max |