Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dalton, D.A.; Diaz del Castillo, L.; Kahn, M.L.; Joyner, S.L.; Chatfield, J.M.
    Heterologous expression and characterization of soybean cytosolic ascorbate peroxidase (1996), Arch. Biochem. Biophys., 328, 1-8.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11 expression in Escherichia coli, most of the enzyme produced is present in the apo-form, without heme Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.02
-
H2O2 recombinant enzyme Glycine max
1.11.1.11 0.35
-
ascorbate recombinant enzyme Glycine max
1.11.1.11 1.8
-
guaiacol recombinant enzyme Glycine max
1.11.1.11 2.4
-
pyrogallol recombinant enzyme Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytosol
-
Glycine max 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 30000
-
x * 30000, SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.11 L-ascorbate + H2O2 Glycine max physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation dehydroascorbate + 2 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Glycine max
-
soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11 using Ni2+-agarose affinity chromatography and gel filtration after reconstitution with hemin Glycine max

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.11.1.11 the apo-recombinant protein is easily converted to the holo-recombinant form by in vitro reconstitution with hemin Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 root nodules Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.11 additional information
-
effects of media amendments Glycine max
1.11.1.11 3 4 affinity purified preparation Glycine max
1.11.1.11 31.7
-
purified recombinant enzyme Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 2,2'-azino-di-(3-ethyl-benzothiazoline-(6)-sulfonic acid) + H2O2 3% relative activity to L-ascorbate Glycine max ? + H2O
-
?
1.11.1.11 guaiacol + H2O2 30.5% activity relative to L-ascorbate Glycine max ?
-
?
1.11.1.11 L-ascorbate + H2O2
-
Glycine max dehydroascorbate + H2O
-
?
1.11.1.11 L-ascorbate + H2O2 physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation Glycine max dehydroascorbate + 2 H2O
-
?
1.11.1.11 pyrogallol + H2O2 723% activity relative to L-ascorbate Glycine max 3-hydroxybenzo-1,2-quinone + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 ? x * 30000, SDS-PAGE Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 5.9 7
-
Glycine max
1.11.1.11 7
-
assay at Glycine max