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Literature summary extracted from

  • Koshiba, T.
    Cytosolic ascorbate peroxidase in seedlings and leaves of maize (Zea mays) (1993), Plant Cell Physiol., 34, 713-721.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.11 iodoacetate potent inhibitor Zea mays
1.11.1.11 KCN 87% inhibition at 1 mM Zea mays
1.11.1.11 NaN3 13% inhibition at 5 mM Zea mays
1.11.1.11 p-chloromercuribenzoate 95% inhibition at 0.05 mM Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.035
-
 H2O2
-
 Zea mays
1.11.1.11 0.38
-
 ascorbate
-
 Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytoplasm
-
 Zea mays 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 28000
-
 gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11
-
 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 coleoptile
-
 Zea mays
-
1.11.1.11 leaf young leaves contain low amounts of enzyme, in mature green leaves, small amounts of the enzyme are distributed in vascular systems, in particular in companion cells Zea mays
-
1.11.1.11 mesocotyl
-
 Zea mays
-
1.11.1.11 root
-
 Zea mays
-
1.11.1.11 seed
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 guaiacol + H2O2 poor electron donor Zea mays ?
-
 ?
1.11.1.11 L-ascorbate + H2O2
-
 Zea mays dehydroascorbate + H2O
-
 ?
1.11.1.11 additional information no activity with NADH Zea mays ?
-
 ?
1.11.1.11 pyrogallol + H2O2 238% activity relative to L-ascorbate Zea mays 3-hydroxybenzo-1,2-quinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 monomer 1 * 28000, SDS-PAGE Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 5 6
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.11 heme one protoheme moiety per molecule Zea mays