BRENDA - Enzyme Database

Purification and some properties of L-ascorbic-acid-specific peroxidase in Euglena gracilis Z

Shigeoka, S.; Nahana, Y.; Kitaoka, S.; Arch. Biochem. Biophys. 201, 121-127 (1980) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.11
Al3+
-
Euglena gracilis
1.11.1.11
Br-
marked inhibition at 1 mM
Euglena gracilis
1.11.1.11
Ca2+
-
Euglena gracilis
1.11.1.11
EDTA
slight inhibition, but when the enzyme is incubated with EDTA 1 mM at 37°C for 3 min in the absence of sucrose and ferrous sulfate there is nearly complete inhibition
Euglena gracilis
1.11.1.11
F-
-
Euglena gracilis
1.11.1.11
Hg2+
complete inhibition at 1 mM
Euglena gracilis
1.11.1.11
I-
-
Euglena gracilis
1.11.1.11
KCN
96.4% inhibition at 1 mM
Euglena gracilis
1.11.1.11
Li+
-
Euglena gracilis
1.11.1.11
Mg2+
-
Euglena gracilis
1.11.1.11
Mn2+
marked inhibition at 1 mM
Euglena gracilis
1.11.1.11
NaN3
91.5% inhibition at 1 mM
Euglena gracilis
1.11.1.11
Ni2+
-
Euglena gracilis
1.11.1.11
Zn2+
-
Euglena gracilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.11
0.056
-
H2O2
substrate: L-ascorbic acid
Euglena gracilis
1.11.1.11
0.22
-
H2O2
substrate: pyrogallol
Euglena gracilis
1.11.1.11
0.41
-
L-ascorbic acid
-
Euglena gracilis
1.11.1.11
9.6
-
pyrogallol
-
Euglena gracilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.1.11
Fe
hemoprotein
Euglena gracilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.11
76000
-
gel filtration
Euglena gracilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.11.1.11
L-ascorbate + H2O2
Euglena gracilis
the enzyme works for protection of cell membrane, by reducing the peroxide compounds generated endogenously from unsaturated fatty acids
dehydroascorbate + 2 H2O
-
Euglena gracilis
?
1.11.1.11
L-ascorbate + H2O2
Euglena gracilis
physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation
dehydroascorbate + 2 H2O
-
Euglena gracilis
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.11.1.11
Euglena gracilis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.11.1.11
using ultracentrifugation, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-150, DEAE-cellulose and CM-cellulose
Euglena gracilis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.11.1.11
2.8
-
purified enzyme, electron donor: glutathione, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
5.8
-
purified enzyme, electron donor: iodide, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
18
-
purified enzyme, electron donor: reductic acid, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
20.3
-
purified enzyme, electron donor: guaiacol, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
132.2
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: cumene hydroperoxide
Euglena gracilis
1.11.1.11
142.1
-
purified enzyme, electron donor: D-araboascorbic acid, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
172.8
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: tert-butyl hydroperoxide
Euglena gracilis
1.11.1.11
185.5
-
purified enzyme, electron donor: pyrogallol, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
254
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: H2O2
Euglena gracilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.11.1.11
cytochrome c + H2O2
no activity
439858
Euglena gracilis
? + H2O
-
-
-
?
1.11.1.11
D-araboascorbic acid + H2O2
56% activity relative to L-ascorbate
439858
Euglena gracilis
dehydroascorbate + H2O
-
439858
Euglena gracilis
?
1.11.1.11
guaiacol + H2O2
8% activity relative to L-ascorbate
439858
Euglena gracilis
?
-
439858
Euglena gracilis
?
1.11.1.11
iodide + H2O2
2.3% activity relative to L-ascorbate
439858
Euglena gracilis
?
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbate + H2O2
tert-butyl hydroperoxide and cumene hydroperoxide also serve as electron acceptor
439858
Euglena gracilis
dehydroascorbate + H2O
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbate + H2O2
the enzyme works for protection of cell membrane, by reducing the peroxide compounds generated endogenously from unsaturated fatty acids
439858
Euglena gracilis
dehydroascorbate + 2 H2O
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbate + H2O2
physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation
439858
Euglena gracilis
dehydroascorbate + 2 H2O
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbic acid + cumene hydroperoxide
-
439858
Euglena gracilis
dehydroascorbate + 1,1-dimethylbenzylalcohol + H2O
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbic acid + tert-butylhydroperoxide
-
439858
Euglena gracilis
dehydroascorbate + tert-butylalcohol
-
439858
Euglena gracilis
?
1.11.1.11
additional information
the activity with glutathione is less than 1.1% of that with L-ascorbic acid, no activity with: cytochrome c, NADH, NADPH, palmitic acid and triose reductone
439858
Euglena gracilis
?
-
-
-
?
1.11.1.11
NADPH + H2O2
no activity
439858
Euglena gracilis
? + H2O
-
-
-
?
1.11.1.11
pyrogallol + H2O2
73.1% activity relative to L-ascorbate
439858
Euglena gracilis
3-hydroxybenzo-1,2-quinone + H2O
-
439858
Euglena gracilis
?
1.11.1.11
reductic acid + H2O2
i.e. 2,3-dihydroxy-2-cyclopenten-1-one, 7.1% activity relative to L-ascorbate
439858
Euglena gracilis
?
-
439858
Euglena gracilis
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.11
32
-
assay at
Euglena gracilis
1.11.1.11
32
34
-
Euglena gracilis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.11.1.11
37
-
maximum activity maintained up to
Euglena gracilis
1.11.1.11
40
-
inactivated after 5 min
Euglena gracilis
1.11.1.11
40
-
preincubated in 30% sucrose and 0.05 mM ferrous sulfate, retains 88.1% activity after 5 min
Euglena gracilis
1.11.1.11
52
-
complete inactivation
Euglena gracilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.11
6.2
-
-
Euglena gracilis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.11.1.11
6
7.9
maximum activity maintained
Euglena gracilis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.11
heme
-
Euglena gracilis
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.11.1.11
0.0018
-
KCN
competitive inhibition against H2O2
Euglena gracilis
1.11.1.11
0.0083
-
KCN
uncompetitive inhibition against L-ascorbic acid
Euglena gracilis
1.11.1.11
0.059
-
NaN3
uncompetitive inhibition against H2O2
Euglena gracilis
1.11.1.11
0.085
-
NaN3
competitive inhibition against L-ascorbic acid
Euglena gracilis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.11
heme
-
Euglena gracilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.11
Al3+
-
Euglena gracilis
1.11.1.11
Br-
marked inhibition at 1 mM
Euglena gracilis
1.11.1.11
Ca2+
-
Euglena gracilis
1.11.1.11
EDTA
slight inhibition, but when the enzyme is incubated with EDTA 1 mM at 37°C for 3 min in the absence of sucrose and ferrous sulfate there is nearly complete inhibition
Euglena gracilis
1.11.1.11
F-
-
Euglena gracilis
1.11.1.11
Hg2+
complete inhibition at 1 mM
Euglena gracilis
1.11.1.11
I-
-
Euglena gracilis
1.11.1.11
KCN
96.4% inhibition at 1 mM
Euglena gracilis
1.11.1.11
Li+
-
Euglena gracilis
1.11.1.11
Mg2+
-
Euglena gracilis
1.11.1.11
Mn2+
marked inhibition at 1 mM
Euglena gracilis
1.11.1.11
NaN3
91.5% inhibition at 1 mM
Euglena gracilis
1.11.1.11
Ni2+
-
Euglena gracilis
1.11.1.11
Zn2+
-
Euglena gracilis
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.11.1.11
0.0018
-
KCN
competitive inhibition against H2O2
Euglena gracilis
1.11.1.11
0.0083
-
KCN
uncompetitive inhibition against L-ascorbic acid
Euglena gracilis
1.11.1.11
0.059
-
NaN3
uncompetitive inhibition against H2O2
Euglena gracilis
1.11.1.11
0.085
-
NaN3
competitive inhibition against L-ascorbic acid
Euglena gracilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.11
0.056
-
H2O2
substrate: L-ascorbic acid
Euglena gracilis
1.11.1.11
0.22
-
H2O2
substrate: pyrogallol
Euglena gracilis
1.11.1.11
0.41
-
L-ascorbic acid
-
Euglena gracilis
1.11.1.11
9.6
-
pyrogallol
-
Euglena gracilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.1.11
Fe
hemoprotein
Euglena gracilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.11
76000
-
gel filtration
Euglena gracilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.11.1.11
L-ascorbate + H2O2
Euglena gracilis
the enzyme works for protection of cell membrane, by reducing the peroxide compounds generated endogenously from unsaturated fatty acids
dehydroascorbate + 2 H2O
-
Euglena gracilis
?
1.11.1.11
L-ascorbate + H2O2
Euglena gracilis
physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation
dehydroascorbate + 2 H2O
-
Euglena gracilis
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.11
using ultracentrifugation, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-150, DEAE-cellulose and CM-cellulose
Euglena gracilis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.11.1.11
2.8
-
purified enzyme, electron donor: glutathione, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
5.8
-
purified enzyme, electron donor: iodide, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
18
-
purified enzyme, electron donor: reductic acid, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
20.3
-
purified enzyme, electron donor: guaiacol, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
132.2
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: cumene hydroperoxide
Euglena gracilis
1.11.1.11
142.1
-
purified enzyme, electron donor: D-araboascorbic acid, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
172.8
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: tert-butyl hydroperoxide
Euglena gracilis
1.11.1.11
185.5
-
purified enzyme, electron donor: pyrogallol, electron acceptor: H2O2
Euglena gracilis
1.11.1.11
254
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: H2O2
Euglena gracilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.11.1.11
cytochrome c + H2O2
no activity
439858
Euglena gracilis
? + H2O
-
-
-
?
1.11.1.11
D-araboascorbic acid + H2O2
56% activity relative to L-ascorbate
439858
Euglena gracilis
dehydroascorbate + H2O
-
439858
Euglena gracilis
?
1.11.1.11
guaiacol + H2O2
8% activity relative to L-ascorbate
439858
Euglena gracilis
?
-
439858
Euglena gracilis
?
1.11.1.11
iodide + H2O2
2.3% activity relative to L-ascorbate
439858
Euglena gracilis
?
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbate + H2O2
tert-butyl hydroperoxide and cumene hydroperoxide also serve as electron acceptor
439858
Euglena gracilis
dehydroascorbate + H2O
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbate + H2O2
the enzyme works for protection of cell membrane, by reducing the peroxide compounds generated endogenously from unsaturated fatty acids
439858
Euglena gracilis
dehydroascorbate + 2 H2O
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbate + H2O2
physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation
439858
Euglena gracilis
dehydroascorbate + 2 H2O
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbic acid + cumene hydroperoxide
-
439858
Euglena gracilis
dehydroascorbate + 1,1-dimethylbenzylalcohol + H2O
-
439858
Euglena gracilis
?
1.11.1.11
L-ascorbic acid + tert-butylhydroperoxide
-
439858
Euglena gracilis
dehydroascorbate + tert-butylalcohol
-
439858
Euglena gracilis
?
1.11.1.11
additional information
the activity with glutathione is less than 1.1% of that with L-ascorbic acid, no activity with: cytochrome c, NADH, NADPH, palmitic acid and triose reductone
439858
Euglena gracilis
?
-
-
-
?
1.11.1.11
NADPH + H2O2
no activity
439858
Euglena gracilis
? + H2O
-
-
-
?
1.11.1.11
pyrogallol + H2O2
73.1% activity relative to L-ascorbate
439858
Euglena gracilis
3-hydroxybenzo-1,2-quinone + H2O
-
439858
Euglena gracilis
?
1.11.1.11
reductic acid + H2O2
i.e. 2,3-dihydroxy-2-cyclopenten-1-one, 7.1% activity relative to L-ascorbate
439858
Euglena gracilis
?
-
439858
Euglena gracilis
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.11
32
-
assay at
Euglena gracilis
1.11.1.11
32
34
-
Euglena gracilis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.11.1.11
37
-
maximum activity maintained up to
Euglena gracilis
1.11.1.11
40
-
inactivated after 5 min
Euglena gracilis
1.11.1.11
40
-
preincubated in 30% sucrose and 0.05 mM ferrous sulfate, retains 88.1% activity after 5 min
Euglena gracilis
1.11.1.11
52
-
complete inactivation
Euglena gracilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.11
6.2
-
-
Euglena gracilis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.11.1.11
6
7.9
maximum activity maintained
Euglena gracilis