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Literature summary extracted from

  • Martinez, M.J.; Ruiz-Duenas, F.J.; Guillen, F.; Martinez, A.T.
    Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii (1996), Eur. J. Biochem., 237, 424-432.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.13 additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Pleurotus ostreatus
1.11.1.13 additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Pleurotus pulmonarius
1.11.1.13 additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Pleurotus eryngii
1.11.1.13 additional information strong increase of MnP levels by growth in presence of peptone, compared with glucose-ammonium tartrate medium Lentinus sajor-caju

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.13 H2O2 25% inhibition at 0.5 mM, 75% inhibition at 1 mM Pleurotus eryngii
1.11.1.13 Mn2+ partial inhibition of oxidation of NAD(P)H at the beginning of the reaction, NADH: 0.1 mM Mn2+ causes 97% inhibition of MP1 and 72% inhibition of MP2 Pleurotus eryngii
1.11.1.13 additional information Mn2+ inhibits MnP synthesis Pleurotus eryngii
1.11.1.13 Sodium metavanadate partially inhibits only peroxidase activity against veratryl alcohol Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.13 0.005
-
 vanillylideneacetone 0.1 mM Mn2+, 0.1 mM H2O2 Pleurotus eryngii
1.11.1.13 0.006 0.01 H2O2 0.1 mM Mn2+ Pleurotus eryngii
1.11.1.13 0.01
-
 2,6-dimethoxyphenol 0.1 mM Mn2+, 0.1 mM H2O2 Pleurotus eryngii
1.11.1.13 0.015
-
 Mn2+ 0.1 mM 2,6-dimethoxyphenol, 0.1 mM H2O2 Pleurotus eryngii
1.11.1.13 0.02
-
 Mn2+ 0.1 mM H2O2 Pleurotus eryngii
1.11.1.13 0.06 0.07 NADH
-
 Pleurotus eryngii
1.11.1.13 0.16 0.25 2,6-dimethoxyphenol 0.1 mM H2O2 Pleurotus eryngii
1.11.1.13 3 3.5 veratryl alcohol 0.1 mM H2O2 Pleurotus eryngii
1.11.1.16 0.005
-
 vanillylidenacetone presence of Mn2+, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
1.11.1.16 0.005
-
 vanillylidenacetone presence of Mn2+, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
1.11.1.16 0.006
-
 H2O2 cosubstrate Mn2+, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
1.11.1.16 0.01
-
 H2O2 cosubstrate Mn2+, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
1.11.1.16 0.01
-
 2,6-dimethoxyphenol presence of Mn2+, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
1.11.1.16 0.01
-
 2,6-dimethoxyphenol presence of Mn2+, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
1.11.1.16 0.015
-
 Mn2+ cosubstrate dimethoxyphenol, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
1.11.1.16 0.015
-
 Mn2+ cosubstrate dimethoxyphenol, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
1.11.1.16 0.02
-
 Mn2+ cosubstrate H2O2, isoenzyme MP-1, pH 5.0 Pleurotus eryngii
1.11.1.16 0.02
-
 Mn2+ cosubstrate H2O2, isoenzyme MP-2, pH 5.0 Pleurotus eryngii
1.11.1.16 0.16
-
 2,6-dimethoxyphenol absence of Mn2+, isoenzyme MP-1, pH 3.0 Pleurotus eryngii
1.11.1.16 0.25
-
 2,6-dimethoxyphenol absence of Mn2+, isoenzyme MP-2, pH 3.0 Pleurotus eryngii
1.11.1.16 3
-
 veratryl alcohol absence of Mn2+, isoenzyme MP-2, pH 3.0 Pleurotus eryngii
1.11.1.16 3.5
-
 veratryl alcohol absence of Mn2+, isoenzyme MP-1, pH 3.0 Pleurotus eryngii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.13 43000
-
 x * 43000, SDS-PAGE Pleurotus ostreatus
1.11.1.13 43000
-
 x * 43000, SDS-PAGE Pleurotus eryngii
1.11.1.16 43000
-
 x * 43000, SDS-PAGE, both isoenzyme MP-1 and MP-2 Pleurotus eryngii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 Pleurotus ostreatus involved in lignin-degradation Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2 Pleurotus eryngii involved in lignin-degradation Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2 Pleurotus ostreatus CBS 411.71 involved in lignin-degradation Mn3+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Lentinus sajor-caju
-
 white rot fungus, strain MUCL 29757
-
1.11.1.13 Pleurotus eryngii
-
 white rot fungus, strain CBS 613.91, 2 isoenzymes: MnP1 and MnP2
-
1.11.1.13 Pleurotus ostreatus
-
-
-
1.11.1.13 Pleurotus ostreatus CBS 411.71
-
-
-
1.11.1.13 Pleurotus pulmonarius
-
 white rot fungus, strain CBS 507.85
-
1.11.1.16 Pleurotus eryngii
-
 isoenzymes MP-1 and MP-2, addition of Mn2+ to growth medium represses enzyme activity
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.11.1.13 higher resistance to H2O2 than MnP from Phanerochaete chrysosporium Pleurotus eryngii

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.13 glycoprotein
-
 Pleurotus ostreatus
1.11.1.13 glycoprotein MP1: up to 5% carbohydrate, MP2: up to 7% carbohydrate Pleurotus eryngii
1.11.1.16 glycoprotein MP-1, up to 5% carbohydrate content, MP-2, up to 7% carbohydrate Pleurotus eryngii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13 25fold purification Pleurotus eryngii
1.11.1.13 MnP1 and MnP2 Pleurotus eryngii
1.11.1.16
-
 Pleurotus eryngii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.13 170
-
 MP2 Pleurotus eryngii
1.11.1.13 187
-
 MP1 Pleurotus eryngii

Storage Stability

EC Number Storage Stability Organism
1.11.1.13 -20°C, stable Pleurotus eryngii
1.11.1.13 4°C, 72 h, stable Pleurotus eryngii
1.11.1.16 -20°C, stable Pleurotus eryngii
1.11.1.16 4°C, stable for at least 72h Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 2,6-dimethoxyphenol + H2O2 + H+
-
 Pleurotus eryngii coerulignone + H2O
-
 r
1.11.1.13 4-(4-hydroxy-3-methoxy-phenyl)-2-butanone + H2O2
-
 Pleurotus eryngii 4-[6,2'-dihydroxy-5,3'-dimethoxy-5'-(3-oxo-butyl)-biphenyl]-butan-2-one + 4-(4-hydroxy-3-methoxyphenyl)-3-buten-2-one + 4-[6,2'-dihydroxy-5,3'-dimethoxy-5'-(3-oxo-butyl)-biphenyl]-3-buten-2-one + 3-(3-oxo-butyl)-hexa-2,4-dienedioic acid-1-methyl ester
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Pleurotus pulmonarius Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Lentinus sajor-caju Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Pleurotus eryngii Mn3+ + H2O Mn3+ oxidizes vanillylideneacetone ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Pleurotus ostreatus Mn3+ + H2O Mn3+ oxidizes 2,6-dimethoxyphenol ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Pleurotus eryngii Mn3+ + H2O Mn3+ oxidizes 2,6-dimethoxyphenol ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Pleurotus eryngii Mn3+ + H2O Mn3+ oxidizes phenol red ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Pleurotus eryngii Mn3+ + H2O Mn3+ oxidizes a variety of phenols ?
1.11.1.13 Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus ostreatus Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus eryngii Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Pleurotus ostreatus CBS 411.71 Mn3+ + H2O Mn3+ oxidizes 2,6-dimethoxyphenol ?
1.11.1.13 Mn2+ + H+ + H2O2 involved in lignin-degradation Pleurotus ostreatus CBS 411.71 Mn3+ + H2O
-
 ?
1.11.1.13 additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus ostreatus ?
-
 ?
1.11.1.13 additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus eryngii ?
-
 ?
1.11.1.13 additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus ostreatus ?
-
 ?
1.11.1.13 additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus eryngii ?
-
 ?
1.11.1.13 additional information Mn2+-independent peroxidase activity against phenolic substrates, e.g. phenol red Pleurotus eryngii ?
-
 ?
1.11.1.13 additional information Mn2+-dependent oxidation of 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus eryngii ?
-
 ?
1.11.1.13 additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus ostreatus ?
-
 ?
1.11.1.13 additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus eryngii ?
-
 ?
1.11.1.13 additional information in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Pleurotus ostreatus CBS 411.71 ?
-
 ?
1.11.1.13 additional information Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol Pleurotus ostreatus CBS 411.71 ?
-
 ?
1.11.1.13 additional information Mn2+-independent oxidase activity on NAD(P)H Pleurotus ostreatus CBS 411.71 ?
-
 ?
1.11.1.13 NADH + H2O2 + H+
-
 Pleurotus eryngii ?
-
 r
1.11.1.13 veratryl alcohol + H2O2 + H+
-
 Pleurotus eryngii ?
-
 r
1.11.1.16 2 2,6-dimethoxyphenol + 2 H2O2 Mn2+-dependent and independent activity Pleurotus eryngii coerulignone + 2 H2O
-
 ?
1.11.1.16 Mn2+ + H2O2
-
 Pleurotus eryngii Mn3+ + H2O
-
 ?
1.11.1.16 NADH + H2O2
-
 Pleurotus eryngii NAD+ + H2O
-
 ?
1.11.1.16 phenol red + H2O2 Mn2+-dependent activity Pleurotus eryngii oxidized phenol red + H2O
-
 ?
1.11.1.16 vanillylidenacetone + H2O2 Mn2+-dependent activity Pleurotus eryngii ? + H2O
-
 ?
1.11.1.16 veratryl alcohol + H2O2 + H+ Mn2+-independent activity Pleurotus eryngii veratraldehyde + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.13 ? x * 43000, SDS-PAGE Pleurotus ostreatus
1.11.1.13 ? x * 43000, SDS-PAGE Pleurotus eryngii
1.11.1.16 ? x * 43000, SDS-PAGE, both isoenzyme MP-1 and MP-2 Pleurotus eryngii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.13 additional information
-
-
 Pleurotus ostreatus
1.11.1.13 additional information
-
 2 isozymes: pI 3.8 and 3.7 Pleurotus eryngii
1.11.1.13 3
-
 oxidation of 2,6-dimethoxyphenol and veratryl alcohol in absence of Mn2+ Pleurotus ostreatus
1.11.1.13 3
-
 oxidation of 2,6-dimethoxyphenol and veratryl alcohol in absence of Mn2+ Pleurotus eryngii
1.11.1.13 3.5
-
 oxidation of NAD(P)H Pleurotus ostreatus
1.11.1.13 3.5
-
 oxidation of NAD(P)H Pleurotus eryngii
1.11.1.13 4
-
-
 Pleurotus ostreatus
1.11.1.13 4
-
 oxidation of 2,6-dimethoxyphenol in presence of Mn2+ Pleurotus eryngii
1.11.1.13 5
-
 oxidation of Mn2+ Pleurotus ostreatus
1.11.1.13 5
-
 oxidation of Mn2+ Pleurotus eryngii
1.11.1.16 3
-
 oxidation of 2,6-dimethoxyphenol, absence of Mn2+ Pleurotus eryngii
1.11.1.16 4
-
 oxidation of 2,6-dimethoxyphenol, presence of Mn2+ Pleurotus eryngii
1.11.1.16 5
-
 oxidation of Mn2+ Pleurotus eryngii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.13 3 5 oxidation of veratryl alcohol decreases more than 95% when the pH is increased from 3 to 4.5 and nearly absent at pH 5 Pleurotus eryngii
1.11.1.13 3 3.5 pH 3.5: decrease of 95% of oxidation of Mn2+, pH 3: nearly no oxidation of Mn2+ Pleurotus eryngii

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.13 heme
-
 Pleurotus ostreatus
1.11.1.13 heme one mol heme per mol of enzyme Pleurotus eryngii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.16 Pleurotus eryngii isoelectric focusing, isoenzyme MP-2
-
 3.65
1.11.1.16 Pleurotus eryngii isoelectric focusing, isoenzyme MP-2
-
 3.75