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Literature summary extracted from

  • Miyazaki, C.; Takahashi, H.
    Engineering of the H2O2-binding pocket region of a recombinant manganese peroxidase to be resistant to H2O2 (2001), FEBS Lett., 509, 111-114.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.13 environmental protection mediated system of degradation is potentially valuable for degradation of synthetic polymers and of environmental pollutants Phanerodontia chrysosporium
1.11.1.13 paper production mediated system of degradation is potentially valuable for pulp and paper industries Phanerodontia chrysosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.13 cDNA encoding MnP2 is cloned and expressed in Escherichia coli BL21(DE3)LysS Phanerodontia chrysosporium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.13 crystal structure Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.13 M237L engineered mutant Phanerodontia chrysosporium
1.11.1.13 M273L mutant with high H2O2 resistance, i.e. 4.1fold higher than that of wild-type, Met-273 is located near the active site pocket and is converted to a non-oxidizable Leu Phanerodontia chrysosporium
1.11.1.13 M67L engineered mutant Phanerodontia chrysosporium
1.11.1.13 N81S mutant enzyme is not inhibited by 1 mM H2O2, H2O2-dependency is 5.5fold higher than that of wild-type, engineering of Asn-81, which might have conformational changes due to the environment of the pocket, to a non-bulky and non-oxidizable Ser Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.13 H2O2 1 mM, complete, irreversible inactivation of wild-type enzyme correlated with the production of methionine sulfoxide, full activity can be retained by engineering Asn-81, which might have conformational changes due to the environment of the pocket, to a non-bulky and non-oxidizable Ser Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Phanerodontia chrysosporium Q02567 MnP2
-
1.11.1.13 Phanerodontia chrysosporium Q02567 strain ATCC 64314
-
1.11.1.13 Phanerodontia chrysosporium MnP2 Q02567 MnP2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.13 glycoprotein native MnP is glycosylated, but not recombinant MnP Phanerodontia chrysosporium

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13 purification of recombinant MnP, expressed in Escherichia coli Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium MnP2 Mn3+ + H2O
-
 ?
1.11.1.13 additional information structural properties Phanerodontia chrysosporium ?
-
 ?
1.11.1.13 additional information enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phanerodontia chrysosporium ?
-
 ?
1.11.1.13 additional information structural properties Phanerodontia chrysosporium MnP2 ?
-
 ?
1.11.1.13 additional information enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phanerodontia chrysosporium MnP2 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.13 additional information
-
 very sensitive to thermal treatment Phanerodontia chrysosporium