EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | alpha-hydroxy acid | activates by chelating and stabilizing Mn3+ rather than activating the enzyme | Dichomitus squalens | |
1.11.1.13 | citrate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
1.11.1.13 | gluconate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
1.11.1.13 | glycolate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
1.11.1.13 | H2O2 | H2O2-dependent | Dichomitus squalens | |
1.11.1.13 | L-malate | activates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
1.11.1.13 | L-Tartrate | stimulates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
1.11.1.13 | Lactate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
1.11.1.13 | Maleate | slightly activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
1.11.1.13 | malonate | stimulates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
1.11.1.13 | additional information | acetate is not an effective chelator | Dichomitus squalens | |
1.11.1.13 | oxalate | activates by chelating and stabilizing Mn3+ | Dichomitus squalens | |
1.11.1.13 | phenylacetate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
1.11.1.13 | phosphate | activates, chelates and stabilizes Mn3+ | Dichomitus squalens | |
1.11.1.13 | succinate | slightly activates, chelates Mn3+ | Dichomitus squalens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.13 | MnP1 and MnP2 are encoded by different genes | Dichomitus squalens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.13 | 0.025 | - |
Mn2+ | MnP2 | Dichomitus squalens | |
1.11.1.13 | 0.039 | - |
Mn2+ | MnP1 | Dichomitus squalens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.13 | extracellular | - |
Dichomitus squalens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | additional information | expression of MnP isoenzymes is dependent on the presence of Mn, expression rather than enzymatic activity is regulated by Mn | Dichomitus squalens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 48000 | - |
MnP1 and MnP2, gel filtration | Dichomitus squalens |
1.11.1.13 | 48900 | - |
1 * 48000, MnP1, 1 * 48900, MnP2, SDS-PAGE | Dichomitus squalens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | Dichomitus squalens | involved in lignin-degradation | Mn3+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.13 | Dichomitus squalens | - |
white rot fungus | - |
1.11.1.13 | Dichomitus squalens | - |
2 isoenzymes: MnP1 and MnP2, syn. Polyporus anceps | - |
1.11.1.13 | Dichomitus squalens CBS 432.34 | - |
2 isoenzymes: MnP1 and MnP2, syn. Polyporus anceps | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.11.1.13 | glycoprotein | neutral carbohydrate content: MnP1: 8.5%, MnP2: 10.3% | Dichomitus squalens |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.13 | 148fold purification of MnP1 and 157fold purification of MnP2 | Dichomitus squalens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 174 | - |
MnP1 | Dichomitus squalens |
1.11.1.13 | 184 | - |
MnP2 | Dichomitus squalens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | specifically oxidizes Mn2+ | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | single Mn2+ binding site in the vicinity of the heme | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | dicarboxylic acids facilitate the dissociation of Mn3+ from enzyme | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-phenylenediamine and p-anisidine | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ to Mn3+ in the presence of organic acid chelators | Dichomitus squalens | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | involved in lignin-degradation | Dichomitus squalens | Mn3+ + H2O | - |
? | |
1.11.1.13 | additional information | no oxidation of Co2+ | Dichomitus squalens | ? | - |
? | |
1.11.1.13 | additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Dichomitus squalens | ? | - |
? | |
1.11.1.13 | additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Dichomitus squalens | ? | - |
? | |
1.11.1.13 | additional information | no activity with veratryl alcohol | Dichomitus squalens | ? | - |
? | |
1.11.1.13 | additional information | no oxidation of Fe2+, Cu2+, Zn2+ | Dichomitus squalens | ? | - |
? | |
1.11.1.13 | additional information | no other metal can substitute Mn2+ | Dichomitus squalens | ? | - |
? | |
1.11.1.13 | additional information | enzyme oxidizes 2,6-dimethoxyphenol | Dichomitus squalens | ? | - |
? | |
1.11.1.13 | additional information | no oxidation of Ni2+ | Dichomitus squalens | ? | - |
? | |
1.11.1.13 | additional information | no oxidation of Co2+ | Dichomitus squalens CBS 432.34 | ? | - |
? | |
1.11.1.13 | additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Dichomitus squalens CBS 432.34 | ? | - |
? | |
1.11.1.13 | additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Dichomitus squalens CBS 432.34 | ? | - |
? | |
1.11.1.13 | additional information | no activity with veratryl alcohol | Dichomitus squalens CBS 432.34 | ? | - |
? | |
1.11.1.13 | additional information | no oxidation of Fe2+, Cu2+, Zn2+ | Dichomitus squalens CBS 432.34 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.13 | monomer | 1 * 48000, MnP1, 1 * 48900, MnP2, SDS-PAGE | Dichomitus squalens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | additional information | - |
assay at room temperature | Dichomitus squalens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | additional information | - |
MnP1: pI 4.1, MnP2: pI 3.9 | Dichomitus squalens |
1.11.1.13 | 4.5 | - |
MnP1 in 50 mM malonate | Dichomitus squalens |
1.11.1.13 | 5 | - |
MnP2 in 50 mM malonate | Dichomitus squalens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | heme | enzyme contains a pentacoordinated, essentially high-spin ferric heme | Dichomitus squalens | |
1.11.1.13 | heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Dichomitus squalens |