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Literature summary extracted from

  • Aitken, M.; Irvine, R.L.
    Characterization of reactions catalyzed by manganese peroxidase from Phanerochaete chrysosporium (1990), Arch. Biochem. Biophys., 276, 405-414.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.13 citrate activates, forms stable Mn3+ complex Phanerodontia chrysosporium
1.11.1.13 H2O2 H2O2-dependent Phanerodontia chrysosporium
1.11.1.13 L-malate activates, forms stable Mn3+ complex Phanerodontia chrysosporium
1.11.1.13 L-Tartrate activates, forms stable Mn3+ complex Phanerodontia chrysosporium
1.11.1.13 Lactate stimulates by complexing with and stabilizing Mn3+ Phanerodontia chrysosporium
1.11.1.13 additional information succinate, formate and acetate do not stabilize Mn3+ Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.13 acetate 100 mM Phanerodontia chrysosporium
1.11.1.13 Cu2+ inhibits by catalyzing the H2O2-dependent reduction of Mn3+ Phanerodontia chrysosporium
1.11.1.13 diphosphate inhibits oxidation of 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phanerodontia chrysosporium
1.11.1.13 formate
-
 Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.13 0.036
-
 Mn2+
-
 Phanerodontia chrysosporium
1.11.1.13 0.057 0.059 H2O2 Mn3+-tartrate formation Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.13 extracellular
-
 Phanerodontia chrysosporium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.13 46000
-
-
 Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 Phanerodontia chrysosporium involved in lignin-degradation Mn3+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Phanerodontia chrysosporium
-
-
-
1.11.1.13 Phanerodontia chrysosporium
-
 white rot basidomycete
-
1.11.1.13 Phanerodontia chrysosporium VKM F-1767
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13
-
 Phanerodontia chrysosporium

Reaction

EC Number Reaction Comment Organism Reaction ID
1.11.1.13 2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O mechanism Phanerodontia chrysosporium
a
1.11.1.13 2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O ping-pong mechanism Phanerodontia chrysosporium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.13 additional information
-
 216-467, 1 unit: initial increase in absorbance of 1.0 per min at 465 nm Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 Mn(III)-tartrate + H2O
-
 Phanerodontia chrysosporium Mn(II)-tartrate + H+ + H2O
-
 r
1.11.1.13 Mn2+ + H+ + H2O2 role of manganese in organic compound oxidations by MnP is to serve as a one-electron transfer mediator Phanerodontia chrysosporium Mn3+ + H2O Mn3+ complex oxidizes a variety of organic substrates ?
1.11.1.13 Mn2+ + H+ + H2O2 role of manganese in organic compound oxidations by MnP is to serve as a one-electron transfer mediator Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes phenolic lignin model compounds ?
1.11.1.13 Mn2+ + H+ + H2O2 role of manganese in organic compound oxidations by MnP is to serve as a one-electron transfer mediator Phanerodontia chrysosporium Mn3+ + H2O Mn3+-chelate-complexes catalyze decarboxylation and demeth(ox)ylation of aromatic substrates ?
1.11.1.13 Mn2+ + H+ + H2O2 role of manganese in organic compound oxidations by MnP is to serve as a one-electron transfer mediator Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes guaiacol ?
1.11.1.13 Mn2+ + H+ + H2O2 involved in lignin-degradation Phanerodontia chrysosporium Mn3+ + H2O
-
 ?
1.11.1.13 Mn2+ + H+ + H2O2 role of manganese in organic compound oxidations by MnP is to serve as a one-electron transfer mediator Phanerodontia chrysosporium VKM F-1767 Mn3+ + H2O Mn3+ complex oxidizes a variety of organic substrates ?
1.11.1.13 Mn2+ + H+ + H2O2 role of manganese in organic compound oxidations by MnP is to serve as a one-electron transfer mediator Phanerodontia chrysosporium VKM F-1767 Mn3+ + H2O Mn3+ oxidizes phenolic lignin model compounds ?
1.11.1.13 Mn2+ + H+ + H2O2 role of manganese in organic compound oxidations by MnP is to serve as a one-electron transfer mediator Phanerodontia chrysosporium VKM F-1767 Mn3+ + H2O Mn3+-chelate-complexes catalyze decarboxylation and demeth(ox)ylation of aromatic substrates ?
1.11.1.13 additional information enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phanerodontia chrysosporium ?
-
 ?
1.11.1.13 additional information catalytic cycle with oxidized intermediates MnP compound I and II Phanerodontia chrysosporium ?
-
 ?
1.11.1.13 additional information enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phanerodontia chrysosporium VKM F-1767 ?
-
 ?
1.11.1.13 additional information catalytic cycle with oxidized intermediates MnP compound I and II Phanerodontia chrysosporium VKM F-1767 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.13 98.3
-
 Mn(III)-tartrate preparation M2 Phanerodontia chrysosporium
1.11.1.13 118
-
 Mn3+-tartrate preparation M1 Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.13 4.8
-
 Mn3+-tartrate and Mn3+-malate formation Phanerodontia chrysosporium