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Literature summary extracted from

  • Mozaffar, S.; Ueda, M.; Kitatsuji, K.; Shimizu, S.; Osumi, M.; Tanaka, A.
    Properties of catalase purified from a methanol-grown yeast, Kloeckera sp. 2201 (1986), Eur. J. Biochem., 155, 527-531.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.6 ammonium sulfate precipitation Kloeckera sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 H2O2 inhibition above 50 mM Kloeckera sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 25
-
 H2O2 catalase activity Kloeckera sp.
1.11.1.6 83
-
 methanol peroxidase activity Kloeckera sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.6 peroxisome
-
 Kloeckera sp. 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 62000
-
 4 * 62000, SDS-PAGE Kloeckera sp.
1.11.1.6 240000
-
 gel filtration Kloeckera sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Kloeckera sp.
-
 O2 + H2O
-
 ?
1.11.1.6 H2O2 Kloeckera sp. 2201
-
 O2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Kloeckera sp.
-
-
-
1.11.1.6 Kloeckera sp. 2201
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 to homogeneity, chromatography steps Kloeckera sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 cell culture methanol grown Kloeckera sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 282000
-
-
 Kloeckera sp.

Storage Stability

EC Number Storage Stability Organism
1.11.1.6 4°C, more than six months Kloeckera sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 beta-(3,4-dihydroxyphenyl)-L-alanine + H2O2 low activity Kloeckera sp. ? + H2O
-
 ?
1.11.1.6 beta-(3,4-dihydroxyphenyl)-L-alanine + H2O2 low activity Kloeckera sp. 2201 ? + H2O
-
 ?
1.11.1.6 H2O2
-
 Kloeckera sp. O2 + H2O
-
 ?
1.11.1.6 H2O2
-
 Kloeckera sp. 2201 O2 + H2O
-
 ?
1.11.1.6 methanol + H2O2 only in the presence of glucose and glucose oxidase Kloeckera sp. formaldehyde + ?
-
 ?
1.11.1.6 methanol + H2O2 only in the presence of glucose and glucose oxidase Kloeckera sp. 2201 formaldehyde + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 62000, SDS-PAGE Kloeckera sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 7.2
-
-
 Kloeckera sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 5 9 activity within Kloeckera sp.