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Literature summary extracted from

  • Shima, S.; Netrusov, A.; Sordel, M.; Wicke, M.; Hartmann, G.C.; Thauer, R.K.
    Purification, characterization, and primary structure of a monofunctional catalase from Methanosarcina barkeri (1999), Arch. Microbiol., 171, 317-323.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-amino-1H-1,2,4-triazole IC50: 80 mM Methanosarcina barkeri
1.11.1.6 KCN IC50: 0.005 mM Methanosarcina barkeri
1.11.1.6 NaN3 IC50: 0.001 mM Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 54000
-
 4 * 54000, SDS-PAGE Methanosarcina barkeri
1.11.1.6 190000
-
 gel filtration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Methanosarcina barkeri monofunctional catalase O2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Methanosarcina barkeri O93662
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 near homogeneity, chromatography techniques Methanosarcina barkeri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 cell culture
-
 Methanosarcina barkeri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 61500
-
-
 Methanosarcina barkeri

Storage Stability

EC Number Storage Stability Organism
1.11.1.6 4°C, stable for one month Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2 monofunctional catalase Methanosarcina barkeri O2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 54000, SDS-PAGE Methanosarcina barkeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 55
-
 stable up to Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme ferro-hemoprotein Methanosarcina barkeri

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.6 0.001
-
 IC50: 0.001 mM Methanosarcina barkeri NaN3
1.11.1.6 0.005
-
 IC50: 0.005 mM Methanosarcina barkeri KCN
1.11.1.6 80
-
 IC50: 80 mM Methanosarcina barkeri 3-amino-1H-1,2,4-triazole