Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schneider, K.; Cammack, R.; Schlegel, H.G.; Hall, D.O.
    The iron-sulphur centres of soluble hydrogenase from Alcaligenes eutrophus (1979), Biochim. Biophys. Acta, 578, 445-461.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.1.2 CdSO4 1 mM, complete inhibition Cupriavidus necator
1.12.1.2 CuCl2 0.2 mM, complete inhibition Cupriavidus necator
1.12.1.2 HgCl2 0.001 mM, complete inhibition Cupriavidus necator
1.12.1.2 iodoacetate 1 mM, 10% inhibition Cupriavidus necator
1.12.1.2 additional information not inhibited by O2, CO and chelating agents Cupriavidus necator
1.12.1.2 p-chloromercuribenzoate 0.15 mM, complete inhibition Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.2 soluble
-
 Cupriavidus necator
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.2 Iron 11.5 iron atoms per enzyme molecule, enzyme contains 2 [4Fe-4S] and 2 [2Fe-2S] clusters Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Cupriavidus necator
-
 formerly Alcaligenes eutrophus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.2
-
 Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 57
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
1.12.1.2 -20°C, 4 mg/ml protein concentration, pH 7.0 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Cupriavidus necator H+ + NADH
-
 ?