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Literature summary extracted from

  • Chen, J.S.; Mortenson, L.E.
    Purification and properties of hydrogenase from Clostridium pasteurianum W5 (1974), Biochim. Biophys. Acta, 371, 283-298.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.051
-
 reduced ferredoxin hydrogen evolution Clostridium pasteurianum
1.12.7.2 6.25
-
 reduced methyl viologen hydrogen evolution Clostridium pasteurianum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe 12 iron atoms per molecule Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 55000
-
 gel filtration Clostridium pasteurianum
1.12.7.2 60500
-
 SDS-PAGE Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Clostridium pasteurianum
-
 H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized ferredoxin Clostridium pasteurianum W5
-
 H+ + reduced ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Clostridium pasteurianum
-
-
-
1.12.7.2 Clostridium pasteurianum W5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 4000
-
 hydrogen evolution, ferredoxin and 1 mM methyl viologen as electron donors Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H+ + reduced methyl viologen
-
 Clostridium pasteurianum H2 + oxidized methyl viologen
-
 r
1.12.7.2 H+ + reduced methyl viologen
-
 Clostridium pasteurianum W5 H2 + oxidized methyl viologen
-
 r
1.12.7.2 H2 + electron acceptor methylene blue as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + electron acceptor benzyl viologen as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + electron acceptor methylene blue as electron acceptor Clostridium pasteurianum W5 H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Clostridium pasteurianum W5 H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + electron acceptor benzyl viologen as electron acceptor Clostridium pasteurianum W5 H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + oxidized ferredoxin
-
 Clostridium pasteurianum H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized ferredoxin
-
 Clostridium pasteurianum W5 H+ + reduced ferredoxin
-
 r