BRENDA - Enzyme Database show

Purification and properties of hydrogenase from Clostridium pasteurianum W5

Chen, J.S.; Mortenson, L.E.; Biochim. Biophys. Acta 371, 283-298 (1974)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.7.2
0.051
-
reduced ferredoxin
hydrogen evolution
Clostridium pasteurianum
1.12.7.2
6.25
-
reduced methyl viologen
hydrogen evolution
Clostridium pasteurianum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.7.2
Fe
12 iron atoms per molecule
Clostridium pasteurianum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.7.2
55000
-
gel filtration
Clostridium pasteurianum
1.12.7.2
60500
-
SDS-PAGE
Clostridium pasteurianum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
Clostridium pasteurianum
-
H+ + reduced ferredoxin
-
Clostridium pasteurianum
r
1.12.7.2
H2 + oxidized ferredoxin
Clostridium pasteurianum W5
-
H+ + reduced ferredoxin
-
Clostridium pasteurianum W5
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.7.2
Clostridium pasteurianum
-
-
-
1.12.7.2
Clostridium pasteurianum W5
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.12.7.2
-
Clostridium pasteurianum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.12.7.2
4000
-
hydrogen evolution, ferredoxin and 1 mM methyl viologen as electron donors
Clostridium pasteurianum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439682
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439682
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439682
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439682
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439682
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439682
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + oxidized ferredoxin
-
439682
Clostridium pasteurianum
H+ + reduced ferredoxin
-
439682
Clostridium pasteurianum
r
1.12.7.2
H2 + oxidized ferredoxin
-
439682
Clostridium pasteurianum W5
H+ + reduced ferredoxin
-
439682
Clostridium pasteurianum W5
r
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.7.2
0.051
-
reduced ferredoxin
hydrogen evolution
Clostridium pasteurianum
1.12.7.2
6.25
-
reduced methyl viologen
hydrogen evolution
Clostridium pasteurianum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.7.2
Fe
12 iron atoms per molecule
Clostridium pasteurianum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.7.2
55000
-
gel filtration
Clostridium pasteurianum
1.12.7.2
60500
-
SDS-PAGE
Clostridium pasteurianum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
Clostridium pasteurianum
-
H+ + reduced ferredoxin
-
Clostridium pasteurianum
r
1.12.7.2
H2 + oxidized ferredoxin
Clostridium pasteurianum W5
-
H+ + reduced ferredoxin
-
Clostridium pasteurianum W5
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.7.2
-
Clostridium pasteurianum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.12.7.2
4000
-
hydrogen evolution, ferredoxin and 1 mM methyl viologen as electron donors
Clostridium pasteurianum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439682
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439682
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439682
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439682
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439682
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439682
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + oxidized ferredoxin
-
439682
Clostridium pasteurianum
H+ + reduced ferredoxin
-
439682
Clostridium pasteurianum
r
1.12.7.2
H2 + oxidized ferredoxin
-
439682
Clostridium pasteurianum W5
H+ + reduced ferredoxin
-
439682
Clostridium pasteurianum W5
r