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Literature summary extracted from

  • Adams, M.W.W.; Mortenson, L.E.
    The purification of hydrogenase II (uptake hydrogenase) from the anaerobic N2-fixing bacterium Clostridium pasteurianum (1984), Biochim. Biophys. Acta, 766, 51-61.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe hydrogenase I contains 12 atoms per mol enzyme and hydrogenase II 8 atoms per mol enzyme Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 53000
-
 hydrogenase II, SDS-PAGE Clostridium pasteurianum

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Clostridium pasteurianum
-
 hydrogenase II, uptake hydrogenase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Clostridium pasteurianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 11
-
 hydrogen production, methylene blue as electron donor, hydrogenase I Clostridium pasteurianum
1.12.7.2 61
-
 hydrogen oxidation, methylene blue as electron acceptor, hydrogenase I Clostridium pasteurianum
1.12.7.2 2998
-
 hydrogen oxidation, methylene blue as electron acceptor, hydrogenase II Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor methylene blue as electron acceptor Clostridium pasteurianum H+ + reduced electron acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.7.2 monomer 1 * 53000, hydrogenase II, SDS-PAGE Clostridium pasteurianum