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The physical and catalytic properties of hydrogenase II of Clostridium pasteurianum. A comparison with hydrogenase I

Adams, M.W.W.; Mortenson, L.E.; J. Biol. Chem. 259, 7045-7055 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.12.7.2
2,2'-bipyridyl
66% activity at 1 mM
Clostridium pasteurianum
1.12.7.2
CO
hydrogenase I is readily inhibited, hydrogenase II is irreversibly inactivated
Clostridium pasteurianum
1.12.7.2
CuSO4
0% activity at 1 mM
Clostridium pasteurianum
1.12.7.2
o-phenanthroline
36% activity at 1 mM
Clostridium pasteurianum
1.12.7.2
O2
50% activity loss after 2 min
Clostridium pasteurianum
1.12.7.2
phenylmethanesulfonyl fluoride
61% activity at 1 mM
Clostridium pasteurianum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.7.2
0.05
-
Ferredoxin
hydrogen evolution, hydrogenase I
Clostridium pasteurianum
1.12.7.2
0.13
-
Ferredoxin
hydrogen evolution, hydrogenase II
Clostridium pasteurianum
1.12.7.2
0.18
-
methylene blue
hydrogen oxidation, hydrogenase I
Clostridium pasteurianum
1.12.7.2
0.31
-
methyl viologen
hydrogen evolution, hydrogenase II
Clostridium pasteurianum
1.12.7.2
0.4
-
methylene blue
hydrogen oxidation, hydrogenase II
Clostridium pasteurianum
1.12.7.2
5
-
methyl viologen
hydrogen oxidation, hydrogenase I
Clostridium pasteurianum
1.12.7.2
5.7
-
methyl viologen
hydrogen oxidation, hydrogenase II
Clostridium pasteurianum
1.12.7.2
6.25
-
methyl viologen
hydrogen evolution, hydrogenase I
Clostridium pasteurianum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.7.2
Fe
hydrogenase I contains 12 atoms per mol enzyme and hydrogenase II 8 atoms per mol enzyme
Clostridium pasteurianum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
Clostridium pasteurianum
-
H+ + reduced ferredoxin
-
Clostridium pasteurianum
r
1.12.7.2
H2 + oxidized ferredoxin
Clostridium pasteurianum W5
-
H+ + reduced ferredoxin
-
Clostridium pasteurianum W5
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.7.2
Clostridium pasteurianum
-
; hydrogenase I, bidirectional hydrogenase; hydrogenase II, uptake hydrogenase
-
1.12.7.2
Clostridium pasteurianum W5
-
-
-
Storage Stability
EC Number
Storage Stability
Organism
1.12.7.2
-180C, 4 months, 100% activity
Clostridium pasteurianum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439669
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439669
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439669
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439669
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + oxidized ferredoxin
-
439669
Clostridium pasteurianum
H+ + reduced ferredoxin
-
439669
Clostridium pasteurianum
r
1.12.7.2
H2 + oxidized ferredoxin
-
439669
Clostridium pasteurianum W5
H+ + reduced ferredoxin
-
439669
Clostridium pasteurianum W5
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.12.7.2
5.8
-
hydrogen evolution, hydrogenase II
Clostridium pasteurianum
1.12.7.2
6.3
-
hydrogen evolution, hydrogenase I
Clostridium pasteurianum
1.12.7.2
7
-
hydrogenase II, hydrogen oxidation, methylene blue as electron acceptor
Clostridium pasteurianum
1.12.7.2
9.1
-
hydrogen evolution, hydrogenase II
Clostridium pasteurianum
1.12.7.2
9.8
-
hydrogenase I, hydrogen oxidation
Clostridium pasteurianum
1.12.7.2
10.5
-
hydrogenase II, hydrogen oxidation, methylene blue as electron acceptor
Clostridium pasteurianum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.12.7.2
2,2'-bipyridyl
66% activity at 1 mM
Clostridium pasteurianum
1.12.7.2
CO
hydrogenase I is readily inhibited, hydrogenase II is irreversibly inactivated
Clostridium pasteurianum
1.12.7.2
CuSO4
0% activity at 1 mM
Clostridium pasteurianum
1.12.7.2
o-phenanthroline
36% activity at 1 mM
Clostridium pasteurianum
1.12.7.2
O2
50% activity loss after 2 min
Clostridium pasteurianum
1.12.7.2
phenylmethanesulfonyl fluoride
61% activity at 1 mM
Clostridium pasteurianum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.7.2
0.05
-
Ferredoxin
hydrogen evolution, hydrogenase I
Clostridium pasteurianum
1.12.7.2
0.13
-
Ferredoxin
hydrogen evolution, hydrogenase II
Clostridium pasteurianum
1.12.7.2
0.18
-
methylene blue
hydrogen oxidation, hydrogenase I
Clostridium pasteurianum
1.12.7.2
0.31
-
methyl viologen
hydrogen evolution, hydrogenase II
Clostridium pasteurianum
1.12.7.2
0.4
-
methylene blue
hydrogen oxidation, hydrogenase II
Clostridium pasteurianum
1.12.7.2
5
-
methyl viologen
hydrogen oxidation, hydrogenase I
Clostridium pasteurianum
1.12.7.2
5.7
-
methyl viologen
hydrogen oxidation, hydrogenase II
Clostridium pasteurianum
1.12.7.2
6.25
-
methyl viologen
hydrogen evolution, hydrogenase I
Clostridium pasteurianum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.7.2
Fe
hydrogenase I contains 12 atoms per mol enzyme and hydrogenase II 8 atoms per mol enzyme
Clostridium pasteurianum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
Clostridium pasteurianum
-
H+ + reduced ferredoxin
-
Clostridium pasteurianum
r
1.12.7.2
H2 + oxidized ferredoxin
Clostridium pasteurianum W5
-
H+ + reduced ferredoxin
-
Clostridium pasteurianum W5
r
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.12.7.2
-180C, 4 months, 100% activity
Clostridium pasteurianum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439669
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439669
Clostridium pasteurianum
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439669
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439669
Clostridium pasteurianum W5
H+ + reduced electron acceptor
-
-
-
-
1.12.7.2
H2 + oxidized ferredoxin
-
439669
Clostridium pasteurianum
H+ + reduced ferredoxin
-
439669
Clostridium pasteurianum
r
1.12.7.2
H2 + oxidized ferredoxin
-
439669
Clostridium pasteurianum W5
H+ + reduced ferredoxin
-
439669
Clostridium pasteurianum W5
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.12.7.2
5.8
-
hydrogen evolution, hydrogenase II
Clostridium pasteurianum
1.12.7.2
6.3
-
hydrogen evolution, hydrogenase I
Clostridium pasteurianum
1.12.7.2
7
-
hydrogenase II, hydrogen oxidation, methylene blue as electron acceptor
Clostridium pasteurianum
1.12.7.2
9.1
-
hydrogen evolution, hydrogenase II
Clostridium pasteurianum
1.12.7.2
9.8
-
hydrogenase I, hydrogen oxidation
Clostridium pasteurianum
1.12.7.2
10.5
-
hydrogenase II, hydrogen oxidation, methylene blue as electron acceptor
Clostridium pasteurianum