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Literature summary extracted from

  • Happe, T.; Naber, J.D.
    Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii (1993), Eur. J. Biochem., 214, 475-481.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.035
-
 oxidized ferredoxin
-
 Chlamydomonas reinhardtii
1.12.7.2 0.83
-
 methyl viologen
-
 Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe 4 atoms per mol enzyme Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 48000
-
 SDS- and native PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Chlamydomonas reinhardtii
-
 H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized ferredoxin Chlamydomonas reinhardtii 137 C(+)
-
 H+ + reduced ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Chlamydomonas reinhardtii
-
-
-
1.12.7.2 Chlamydomonas reinhardtii 137 C(+)
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.7.2 rapid inactivation under aerobic conditions Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 935
-
 hydrogen production, reduced methyl viologen as electron donor Chlamydomonas reinhardtii

Storage Stability

EC Number Storage Stability Organism
1.12.7.2 -80°C, several weeks, strictly anaerobical storage Chlamydomonas reinhardtii
1.12.7.2 5°C, 5 d, strictly anaerobical storage Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H+ + reduced methyl viologen
-
 Chlamydomonas reinhardtii H2 + oxidized methyl viologen
-
 ?
1.12.7.2 H+ + reduced methyl viologen
-
 Chlamydomonas reinhardtii 137 C(+) H2 + oxidized methyl viologen
-
 ?
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Chlamydomonas reinhardtii H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Chlamydomonas reinhardtii 137 C(+) H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + oxidized ferredoxin
-
 Chlamydomonas reinhardtii H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized ferredoxin
-
 Chlamydomonas reinhardtii 137 C(+) H+ + reduced ferredoxin
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 60
-
 hydrogen evolution Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.7.2 6.9
-
 hydrogen evolution Chlamydomonas reinhardtii