Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dobrindt, U.; Blaut, M.
    Purification and characterization of a membrane-bound hydrogenase from Sporomusa sphaeroides involved in energy-transducing electron transport (1996), Arch. Microbiol., 165, 141-147.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.341
-
 H2
-
 Sporomusa sphaeroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.7.2 membrane cytoplasmic Sporomusa sphaeroides 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe 15.6 mol iron per mol enzyme Sporomusa sphaeroides
1.12.7.2 Ni 0.6 mol per mol enzyme Sporomusa sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 37000
-
 1 * 65000 + 1 * 37000, SDS-PAGE Sporomusa sphaeroides
1.12.7.2 65000
-
 1 * 65000 + 1 * 37000, SDS-PAGE Sporomusa sphaeroides
1.12.7.2 122000
-
 native PAGE Sporomusa sphaeroides
1.12.7.2 130000
-
 gel filtration Sporomusa sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Sporomusa sphaeroides
-
 strain DSM 2875
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 purified under aerobic conditions, requires reductive activation Sporomusa sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 19.8
-
 hydrogen oxidation, benzyl viologen as electron acceptor Sporomusa sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor cytochrome b as electron acceptor Sporomusa sphaeroides H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Sporomusa sphaeroides H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor benzyl viologen as electron acceptor Sporomusa sphaeroides H+ + reduced electron acceptor
-
 r

Subunits

EC Number Subunits Comment Organism
1.12.7.2 dimer 1 * 65000 + 1 * 37000, SDS-PAGE Sporomusa sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 65
-
-
 Sporomusa sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.7.2 0.683
-
 H2
-
 Sporomusa sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.7.2 8
-
-
 Sporomusa sphaeroides