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Literature summary extracted from

  • Sapra, R.; Verhagen, M.F.; Adams, M.W.
    Purification and characterization of a membrane-bound hydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus (2000), J. Bacteriol., 182, 3423-3428.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.7.2 O2 50% loss of H2 evolution activity after 3 h Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.7.2 membrane
-
 Pyrococcus furiosus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe 4 Fe-atoms per mol hydrogenase Pyrococcus furiosus
1.12.7.2 Ni 1 Ni atom per mol, redox active Ni-site Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 20000
-
 alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 40000
-
 alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Pyrococcus furiosus
-
 strain DSM 3638
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.7.2 rapid inactivation under aerobic conditions Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 under anaerobic conditions, solubilized with n-dodecyl-beta-D-maltoside Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 0.004
-
 hydrogen oxidation, benzyl viologen as electron acceptor Pyrococcus furiosus
1.12.7.2 9.4
-
 hydrogen evolution, reduced methyl viologen as electron donor Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor benzyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor NADP+ as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
1.12.7.2 additional information ferredoxin is not an efficient electron carrier for both hydrogenases Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.7.2 dimer alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.7.2 100
-
 50% inactivation after 30 min Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.7.2 NADPH
-
 Pyrococcus furiosus