Literature summary extracted from

Hatchikian, E.C.; Forget, N.; Fernandez, V.M.; Williams, R.; Cammack, R.
Further characterization of the [iron]-hydrogenase from Desulfovibrio desulfuricans ATCC 7757 (1992), Eur. J. Biochem., 209, 357-365.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.12.99.6	CO	-	Desulfovibrio desulfuricans
1.12.99.6	Cu2+	65% loss of activity after 2 min at 0.005 mM, complete inhibition at 0.1 mM in the presence of ascorbate, no inhibition observed without ascorbate	Desulfovibrio desulfuricans
1.12.99.6	diethyldicarbonate	65% loss of activity 6 mM	Desulfovibrio desulfuricans
1.12.99.6	nitrite	70% loss of activity after 2 min at 0.04 mM	Desulfovibrio desulfuricans

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.12.99.6	Fe	14 atoms per molecule, 2 4Fe-4S clusters	Desulfovibrio desulfuricans
1.12.99.6	Sulfide	14.4 atoms per molecule	Desulfovibrio desulfuricans

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.12.99.6	11000	-	1 * 42500 + 1 * 11000, SDS-PAGE	Desulfovibrio desulfuricans
1.12.99.6	42500	-	1 * 42500 + 1 * 11000, SDS-PAGE	Desulfovibrio desulfuricans
1.12.99.6	53500	-	analytical ultracentrifugation	Desulfovibrio desulfuricans

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.12.99.6	Desulfovibrio desulfuricans	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.12.99.6	-	Desulfovibrio desulfuricans

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.12.99.6	additional information	-	specific activities with various electron donors	Desulfovibrio desulfuricans

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.12.99.6	H2 + acceptor	methyl viologen acts as electron acceptor	Desulfovibrio desulfuricans	H+ + reduced acceptor	-	r

Subunits

EC Number	Subunits	Comment	Organism
1.12.99.6	dimer	1 * 42500 + 1 * 11000, SDS-PAGE	Desulfovibrio desulfuricans

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.12.99.6	0.00016	-	CO	-	Desulfovibrio desulfuricans

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Release 2023.1 (January 2023)