BRENDA - Enzyme Database

Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction

Ma, K.; Weiss, R.; Adams, M.W.W.; J. Bacteriol. 182, 1864-1871 (2000)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.7.2
0.017
-
NADP+
-
Pyrococcus furiosus
1.12.7.2
0.07
-
NADH
-
Pyrococcus furiosus
1.12.7.2
0.07
-
NADPH
-
Pyrococcus furiosus
1.12.7.2
0.125
-
NAD+
-
Pyrococcus furiosus
1.12.7.2
0.2
-
sulfur
-
Pyrococcus furiosus
1.12.7.2
1
-
methyl viologen
at 120 kPa H2
Pyrococcus furiosus
1.12.7.2
1.25
-
reduced methyl viologen
-
Pyrococcus furiosus
1.12.99.6
additional information
-
additional information
Km for various electron donors
Pyrococcus furiosus
1.12.99.6
0.128
-
NAD+
-
Pyrococcus furiosus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.12.1.5
cytoplasm
-
Pyrococcus furiosus
5737
-
1.12.7.2
cytoplasm
-
Pyrococcus furiosus
5737
-
1.12.98.4
cytoplasm
-
Pyrococcus furiosus
5737
-
1.12.99.6
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.1.5
Fe2+
contains 21 atoms iron per mol enzyme
Pyrococcus furiosus
1.12.1.5
Ni2+
contains 0.9 atoms nickel per mol enzyme
Pyrococcus furiosus
1.12.7.2
Fe
21 atoms per mol heterotetramer; five [4Fe4S] clusters, 2 in the beta subunit and 3 in the delta subunit, additionally one [2Fe2S] cluster in the gamma subunit
Pyrococcus furiosus
1.12.7.2
Ni
0.9 atoms per mol heterotetramer, NiFe-catalytic site in the alpha-subunit
Pyrococcus furiosus
1.12.98.4
Fe2+
contains 21 atoms iron per mol enzyme
Pyrococcus furiosus
1.12.98.4
Ni2+
contains 0.9 atoms nickel per mol enzyme
Pyrococcus furiosus
1.12.99.6
Fe
21 atoms per mol enzyme, 5 4Fe-4S cluster and 1 2Fe-2S cluster
Pyrococcus furiosus
1.12.99.6
Ni
0.9 atoms per mol enzyme
Pyrococcus furiosus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.1.5
24000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.1.5
30000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.1.5
39000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.1.5
52000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.1.5
320000
-
gel filtration
Pyrococcus furiosus
1.12.7.2
24000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
30000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
39000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
52000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
320000
-
gel filtration
Pyrococcus furiosus
1.12.98.4
24000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
30000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
39000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
52000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
320000
-
gel filtration
Pyrococcus furiosus
1.12.99.6
24000
-
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
30000
-
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
39000
-
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
52000
-
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
320000
-
gel filtration
Pyrococcus furiosus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.1.5
Pyrococcus furiosus
E7FHC4
subunit alpha
-
1.12.1.5
Pyrococcus furiosus
E7FHF8
subunit delta
-
1.12.1.5
Pyrococcus furiosus
E7FHN9
subunit beta
-
1.12.1.5
Pyrococcus furiosus
E7FHW8
subunit gamma
-
1.12.1.5
Pyrococcus furiosus
-
-
-
1.12.7.2
Pyrococcus furiosus
-
hydrogenase II; strain DSM 3638
-
1.12.98.4
Pyrococcus furiosus
E7FHC4 and E7FHN9 and E7FHW8 and E7FHF8
subunits alpha, beta, gamma and delta
-
1.12.99.6
Pyrococcus furiosus
-
strain DSM 3638
-
Purification (Commentary)
EC Number
Commentary
Organism
1.12.1.5
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Pyrococcus furiosus
1.12.98.4
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Pyrococcus furiosus
1.12.99.6
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.7.2
0.02
-
hydrogen production, oxidized ferredoxin as electron donor, hydrogenase II
Pyrococcus furiosus
1.12.7.2
0.2
-
sulfur reduction, hydrogenase II
Pyrococcus furiosus
1.12.7.2
0.98
-
hydrogen production, sodium dithionite as electron donor, hydrogenase II
Pyrococcus furiosus
1.12.7.2
11
-
hydrogen oxidation, methyl viologen as electron acceptor, hydrogenase II
Pyrococcus furiosus
1.12.7.2
40
-
hydrogen production, methyl viologen as electron donor, hydrogenase II
Pyrococcus furiosus
1.12.7.2
131
-
hydrogen oxidation, benzyl viologen as electron acceptor, hydrogenase II
Pyrococcus furiosus
1.12.99.6
additional information
-
specific activities with various electron donors
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.5
H2 + NAD+
-
439641
Pyrococcus furiosus
H+ + NADH
-
-
-
r
1.12.1.5
H2 + NADP+
-
439641
Pyrococcus furiosus
H+ + NADPH
-
-
-
r
1.12.1.5
additional information
ferredoxin from Pyrococcus furiosus is not an efficient electron carrier
439641
Pyrococcus furiosus
?
-
-
-
-
1.12.7.2
H+ + reduced methyl viologen
-
439641
Pyrococcus furiosus
H2 + oxidized methyl viologen
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439641
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439641
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
NADP+ as electron acceptor
439641
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
additional information
ferredoxin is not an efficient electron carrier for both hydrogenases
439641
Pyrococcus furiosus
?
-
-
-
-
1.12.7.2
S + NADPH
-
439641
Pyrococcus furiosus
H2S + NADP+
-
439641
Pyrococcus furiosus
?
1.12.98.4
H2 + benzyl viologen
highest activity
439641
Pyrococcus furiosus
H2S + reduced benzyl viologen
-
-
-
?
1.12.98.4
H2 + methyl viologen
-
439641
Pyrococcus furiosus
H2S + reduced methyl viologen
-
-
-
?
1.12.98.4
H2 + polysulfide(n)
-
439641
Pyrococcus furiosus
H2S + polysulfide(n-1)
-
-
-
r
1.12.98.4
additional information
ferredoxin from Pyrococcus furiosus is not an efficient electron carrier
439641
Pyrococcus furiosus
?
-
-
-
-
1.12.98.4
NADPH + H+ + polysulfide(n)
-
439641
Pyrococcus furiosus
NADP+ + polysulfide(n-1)
-
-
-
r
1.12.99.6
H2 + acceptor
benzyl viologen, methyl viologen, NADPH and NADH can serve as electron donors
439641
Pyrococcus furiosus
H+ + reduced acceptor
-
-
-
-
1.12.99.6
H2 + S
-
439641
Pyrococcus furiosus
H2S
-
439641
Pyrococcus furiosus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.12.1.5
heterotetramer
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
octamer
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
heterotetramer
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
tetramer
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.12.7.2
90
-
above 90°C, hydrogenase II
Pyrococcus furiosus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.12.7.2
95
-
50% inactivation after 6 h, hydrogenase II
Pyrococcus furiosus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.12.99.6
additional information
-
additional information
turnover number for various electron donors
Pyrococcus furiosus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.5
FAD
contains 0.83 FAD per mol enzyme
Pyrococcus furiosus
1.12.1.5
NADPH
-
Pyrococcus furiosus
1.12.1.5
[2Fe-2S]-center
contains 1 [2Fe-2S]-center
Pyrococcus furiosus
1.12.1.5
[4Fe-4S]-center
contains 2 [4Fe-4S]-centers
Pyrococcus furiosus
1.12.7.2
FAD
hydrogenase II, 0.83 mol per mol heterotetramer; putative nucleotide-binding site in the gamma subunit
Pyrococcus furiosus
1.12.7.2
NADH
putative nucleotide-binding site in the gamma subunit
Pyrococcus furiosus
1.12.7.2
NADPH
putative nucleotide-binding site in the gamma subunit
Pyrococcus furiosus
1.12.98.4
FAD
contains 0.83 FAD per mol enzyme
Pyrococcus furiosus
1.12.98.4
NADH
NADPH and NADH are equally efficient as electron donors for H2 production
Pyrococcus furiosus
1.12.98.4
NADPH
NADPH and NADH are equally efficient as electron donors for H2 production
Pyrococcus furiosus
1.12.98.4
[2Fe-2S]-center
contains 1 [2Fe-2S] center
Pyrococcus furiosus
1.12.98.4
[4Fe-4S]-center
contains 2 [4Fe-4S]-centers
Pyrococcus furiosus
1.12.99.6
FAD
0.83 mol FAD per enzyme
Pyrococcus furiosus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.12.1.5
FAD
contains 0.83 FAD per mol enzyme
Pyrococcus furiosus
1.12.1.5
NADPH
-
Pyrococcus furiosus
1.12.1.5
[2Fe-2S]-center
contains 1 [2Fe-2S]-center
Pyrococcus furiosus
1.12.1.5
[4Fe-4S]-center
contains 2 [4Fe-4S]-centers
Pyrococcus furiosus
1.12.7.2
FAD
hydrogenase II, 0.83 mol per mol heterotetramer; putative nucleotide-binding site in the gamma subunit
Pyrococcus furiosus
1.12.7.2
NADH
putative nucleotide-binding site in the gamma subunit
Pyrococcus furiosus
1.12.7.2
NADPH
putative nucleotide-binding site in the gamma subunit
Pyrococcus furiosus
1.12.98.4
FAD
contains 0.83 FAD per mol enzyme
Pyrococcus furiosus
1.12.98.4
NADH
NADPH and NADH are equally efficient as electron donors for H2 production
Pyrococcus furiosus
1.12.98.4
NADPH
NADPH and NADH are equally efficient as electron donors for H2 production
Pyrococcus furiosus
1.12.98.4
[2Fe-2S]-center
contains 1 [2Fe-2S] center
Pyrococcus furiosus
1.12.98.4
[4Fe-4S]-center
contains 2 [4Fe-4S]-centers
Pyrococcus furiosus
1.12.99.6
FAD
0.83 mol FAD per enzyme
Pyrococcus furiosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.7.2
0.017
-
NADP+
-
Pyrococcus furiosus
1.12.7.2
0.07
-
NADH
-
Pyrococcus furiosus
1.12.7.2
0.07
-
NADPH
-
Pyrococcus furiosus
1.12.7.2
0.125
-
NAD+
-
Pyrococcus furiosus
1.12.7.2
0.2
-
sulfur
-
Pyrococcus furiosus
1.12.7.2
1
-
methyl viologen
at 120 kPa H2
Pyrococcus furiosus
1.12.7.2
1.25
-
reduced methyl viologen
-
Pyrococcus furiosus
1.12.99.6
additional information
-
additional information
Km for various electron donors
Pyrococcus furiosus
1.12.99.6
0.128
-
NAD+
-
Pyrococcus furiosus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.12.1.5
cytoplasm
-
Pyrococcus furiosus
5737
-
1.12.7.2
cytoplasm
-
Pyrococcus furiosus
5737
-
1.12.98.4
cytoplasm
-
Pyrococcus furiosus
5737
-
1.12.99.6
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.1.5
Fe2+
contains 21 atoms iron per mol enzyme
Pyrococcus furiosus
1.12.1.5
Ni2+
contains 0.9 atoms nickel per mol enzyme
Pyrococcus furiosus
1.12.7.2
Fe
21 atoms per mol heterotetramer; five [4Fe4S] clusters, 2 in the beta subunit and 3 in the delta subunit, additionally one [2Fe2S] cluster in the gamma subunit
Pyrococcus furiosus
1.12.7.2
Ni
0.9 atoms per mol heterotetramer, NiFe-catalytic site in the alpha-subunit
Pyrococcus furiosus
1.12.98.4
Fe2+
contains 21 atoms iron per mol enzyme
Pyrococcus furiosus
1.12.98.4
Ni2+
contains 0.9 atoms nickel per mol enzyme
Pyrococcus furiosus
1.12.99.6
Fe
21 atoms per mol enzyme, 5 4Fe-4S cluster and 1 2Fe-2S cluster
Pyrococcus furiosus
1.12.99.6
Ni
0.9 atoms per mol enzyme
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.1.5
24000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.1.5
30000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.1.5
39000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.1.5
52000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.1.5
320000
-
gel filtration
Pyrococcus furiosus
1.12.7.2
24000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
30000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
39000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
52000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
320000
-
gel filtration
Pyrococcus furiosus
1.12.98.4
24000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
30000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
39000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
52000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
320000
-
gel filtration
Pyrococcus furiosus
1.12.99.6
24000
-
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
30000
-
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
39000
-
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
52000
-
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
320000
-
gel filtration
Pyrococcus furiosus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.1.5
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Pyrococcus furiosus
1.12.98.4
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Pyrococcus furiosus
1.12.99.6
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.7.2
0.02
-
hydrogen production, oxidized ferredoxin as electron donor, hydrogenase II
Pyrococcus furiosus
1.12.7.2
0.2
-
sulfur reduction, hydrogenase II
Pyrococcus furiosus
1.12.7.2
0.98
-
hydrogen production, sodium dithionite as electron donor, hydrogenase II
Pyrococcus furiosus
1.12.7.2
11
-
hydrogen oxidation, methyl viologen as electron acceptor, hydrogenase II
Pyrococcus furiosus
1.12.7.2
40
-
hydrogen production, methyl viologen as electron donor, hydrogenase II
Pyrococcus furiosus
1.12.7.2
131
-
hydrogen oxidation, benzyl viologen as electron acceptor, hydrogenase II
Pyrococcus furiosus
1.12.99.6
additional information
-
specific activities with various electron donors
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.1.5
H2 + NAD+
-
439641
Pyrococcus furiosus
H+ + NADH
-
-
-
r
1.12.1.5
H2 + NADP+
-
439641
Pyrococcus furiosus
H+ + NADPH
-
-
-
r
1.12.1.5
additional information
ferredoxin from Pyrococcus furiosus is not an efficient electron carrier
439641
Pyrococcus furiosus
?
-
-
-
-
1.12.7.2
H+ + reduced methyl viologen
-
439641
Pyrococcus furiosus
H2 + oxidized methyl viologen
-
-
-
-
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439641
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
benzyl viologen as electron acceptor
439641
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
NADP+ as electron acceptor
439641
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
additional information
ferredoxin is not an efficient electron carrier for both hydrogenases
439641
Pyrococcus furiosus
?
-
-
-
-
1.12.7.2
S + NADPH
-
439641
Pyrococcus furiosus
H2S + NADP+
-
439641
Pyrococcus furiosus
?
1.12.98.4
H2 + benzyl viologen
highest activity
439641
Pyrococcus furiosus
H2S + reduced benzyl viologen
-
-
-
?
1.12.98.4
H2 + methyl viologen
-
439641
Pyrococcus furiosus
H2S + reduced methyl viologen
-
-
-
?
1.12.98.4
H2 + polysulfide(n)
-
439641
Pyrococcus furiosus
H2S + polysulfide(n-1)
-
-
-
r
1.12.98.4
additional information
ferredoxin from Pyrococcus furiosus is not an efficient electron carrier
439641
Pyrococcus furiosus
?
-
-
-
-
1.12.98.4
NADPH + H+ + polysulfide(n)
-
439641
Pyrococcus furiosus
NADP+ + polysulfide(n-1)
-
-
-
r
1.12.99.6
H2 + acceptor
benzyl viologen, methyl viologen, NADPH and NADH can serve as electron donors
439641
Pyrococcus furiosus
H+ + reduced acceptor
-
-
-
-
1.12.99.6
H2 + S
-
439641
Pyrococcus furiosus
H2S
-
439641
Pyrococcus furiosus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.12.1.5
heterotetramer
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
octamer
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.98.4
heterotetramer
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
tetramer
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.12.7.2
90
-
above 90°C, hydrogenase II
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.12.7.2
95
-
50% inactivation after 6 h, hydrogenase II
Pyrococcus furiosus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.12.99.6
additional information
-
additional information
turnover number for various electron donors
Pyrococcus furiosus