EC Number | Cloned (Comment) | Organism |
---|---|---|
1.12.1.2 | - |
Cupriavidus necator |
1.12.99.6 | - |
Cupriavidus necator |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.12.1.2 | C461A | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive | Cupriavidus necator |
1.12.1.2 | D456N | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
1.12.1.2 | D456S | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
1.12.1.2 | E14Q | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive | Cupriavidus necator |
1.12.1.2 | E14V | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
1.12.1.2 | G15A | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
1.12.1.2 | H16L | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | H396L | site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | H396Q | site-directed mutagenesis, catalytic subunit HoxH allele, about 3.5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | H69Q | site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | H69Q/P390A | site-directed mutagenesis, catalytic subunit HoxH alleles, reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | I64A | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | L118A | site-directed mutagenesis, catalytic subunit HoxH allele, reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | L118F | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow at O2 concentrations above 5%, about 4fold reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | L118I | site-directed mutagenesis, catalytic subunit HoxH allele, reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | L394A | site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | L394N | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, nearly inactive | Cupriavidus necator |
1.12.1.2 | additional information | construction of an inactive null mutant strain, mutant strain growth characteristics, overview | Cupriavidus necator |
1.12.1.2 | P390A | site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | R12L | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | R12Q | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | R391L | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive | Cupriavidus necator |
1.12.1.2 | R391Q | site-directed mutagenesis, catalytic subunit HoxH allele, mutant strain does not grow, inactive | Cupriavidus necator |
1.12.1.2 | R60Q | site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | S68V | site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | T415S | site-directed mutagenesis, catalytic subunit HoxH allele, about 5fold reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | T415V | site-directed mutagenesis, catalytic subunit HoxH allele, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.1.2 | T415V/N415H | site-directed mutagenesis, catalytic subunit HoxH alleles, O2-sensitive growth, highly reduced activity compared to the wild-type strain | Cupriavidus necator |
1.12.99.6 | additional information | site directed mutagenesis in conserved motifs of the subunit HoxH | Cupriavidus necator |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.1.2 | cytosol | - |
Cupriavidus necator | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | Cupriavidus necator | - |
H+ + NADH | - |
? | |
1.12.1.2 | additional information | Cupriavidus necator | activity is equally high in atmosperes of 20% O2, 20% N2, or 100% H2, the latter being slightly preferred | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.1.2 | Cupriavidus necator | - |
ATCC 17699, DSM 428 | - |
1.12.99.6 | Cupriavidus necator | - |
wild type and mutants | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.12.1.2 | H2 + NAD+ = H+ + NADH | molecular mechanism of hydrogen activation | Cupriavidus necator |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.1.2 | H2 + NAD+ | - |
Cupriavidus necator | H+ + NADH | - |
? | |
1.12.1.2 | H2 + oxidized benzyl viologen | - |
Cupriavidus necator | H+ + reduced benzyl viologen | - |
? | |
1.12.1.2 | additional information | activity is equally high in atmosperes of 20% O2, 20% N2, or 100% H2, the latter being slightly preferred | Cupriavidus necator | ? | - |
? | |
1.12.1.2 | additional information | deuterium-hydrogen proton exchange activity of wild-type and mutantenzymes, overview | Cupriavidus necator | ? | - |
? | |
1.12.99.6 | H2 + acceptor | - |
Cupriavidus necator | H+ + reduced acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.1.2 | tetramer | the catalytic subunit is termed HoxH | Cupriavidus necator |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.1.2 | NAD+-reducing hydrogenase | - |
Cupriavidus necator |
1.12.1.2 | SH | - |
Cupriavidus necator |
1.12.1.2 | soluble hydrogenase | - |
Cupriavidus necator |
1.12.1.2 | [NiFe] hydrogenase | - |
Cupriavidus necator |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.1.2 | 7 | - |
assay at | Cupriavidus necator |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.1.2 | benzyl viologen | oxidized and reduced | Cupriavidus necator | |
1.12.1.2 | NAD+ | - |
Cupriavidus necator | |
1.12.1.2 | NADH | - |
Cupriavidus necator |