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Literature summary extracted from

  • Bryant, F.O.; Adams, M.W.W.
    Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus (1989), J. Biol. Chem., 264, 5070-5079.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.7.2 O2 reversible inhibition Pyrococcus furiosus
1.12.99.6 O2 reversible Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.99.6 0.08
-
 H2 with methylene blue as electron acceptor Pyrococcus furiosus
1.12.99.6 1.9
-
 H2 with methyl viologen as electron acceptor Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 cytoplasm
-
 Pyrococcus furiosus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe 31 atoms per mol Pyrococcus furiosus
1.12.7.2 Ni 0.98 atoms per mol enzyme Pyrococcus furiosus
1.12.99.6 Fe 31 g iron per 185 g enzyme Pyrococcus furiosus
1.12.99.6 Ni 31 g nickel per 185 g enzyme Pyrococcus furiosus
1.12.99.6 Sulfide 24 g acid labile sulfide per 185 g enzyme Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 24000
-
 alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 27000
-
 alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 46000
-
 alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 185000
-
 gel filtration Pyrococcus furiosus
1.12.99.6 24000
-
 alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 27000
-
 alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 46000
-
 alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 185000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Pyrococcus furiosus
-
 H+ + reduced ferredoxin
-
 r
1.12.99.6 H2 + acceptor Pyrococcus furiosus ferredoxin acts as physiological electron donor H+ + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Pyrococcus furiosus
-
 strain DSM 3638
-
1.12.99.6 Pyrococcus furiosus
-
 strain DSM 3638
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Pyrococcus furiosus
1.12.99.6 apparent homogeneity Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.99.6 360
-
-
 Pyrococcus furiosus

Storage Stability

EC Number Storage Stability Organism
1.12.99.6 -196°C no loss of activity after freezing and thawing under argon Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor methylene blue as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + oxidized ferredoxin
-
 Pyrococcus furiosus H+ + reduced ferredoxin
-
 r
1.12.99.6 H2 + acceptor methyl viologen acts as electron acceptor Pyrococcus furiosus H+ + reduced acceptor
-
 r
1.12.99.6 H2 + acceptor methylene blue acts as electron acceptor Pyrococcus furiosus H+ + reduced acceptor
-
 r
1.12.99.6 H2 + acceptor reduced ferredoxin can act as acceptor Pyrococcus furiosus H+ + reduced acceptor
-
 r
1.12.99.6 H2 + acceptor ferredoxin acts as physiological electron donor Pyrococcus furiosus H+ + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.7.2 hexamer alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 hexamer alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 95
-
 hydrogen evolution Pyrococcus furiosus
1.12.7.2 95
-
 above 95°C, hydrogen oxidation Pyrococcus furiosus
1.12.99.6 95
-
 above Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.7.2 100
-
 most of activity is retained after 1 h incubation Pyrococcus furiosus
1.12.99.6 23
-
 no loss of activity after 2 months under anaerobic conditions Pyrococcus furiosus
1.12.99.6 80
-
 50% loss of activity after 23 h Pyrococcus furiosus
1.12.99.6 90
-
 50% loss of activity after 3 h Pyrococcus furiosus
1.12.99.6 100
-
 50% loss of activity after 2 h Pyrococcus furiosus
1.12.99.6 105
-
 50% loss of activity after 5 min Pyrococcus furiosus