BRENDA - Enzyme Database show

Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus

Bryant, F.O.; Adams, M.W.W.; J. Biol. Chem. 264, 5070-5079 (1989)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.12.7.2
O2
reversible inhibition
Pyrococcus furiosus
1.12.99.6
O2
reversible
Pyrococcus furiosus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.99.6
0.08
-
H2
with methylene blue as electron acceptor
Pyrococcus furiosus
1.12.99.6
1.9
-
H2
with methyl viologen as electron acceptor
Pyrococcus furiosus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.12.99.6
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.7.2
Fe
31 atoms per mol
Pyrococcus furiosus
1.12.7.2
Ni
0.98 atoms per mol enzyme
Pyrococcus furiosus
1.12.99.6
Fe
31 g iron per 185 g enzyme
Pyrococcus furiosus
1.12.99.6
Ni
31 g nickel per 185 g enzyme
Pyrococcus furiosus
1.12.99.6
Sulfide
24 g acid labile sulfide per 185 g enzyme
Pyrococcus furiosus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.7.2
24000
-
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
27000
-
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
46000
-
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
185000
-
gel filtration
Pyrococcus furiosus
1.12.99.6
24000
-
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
27000
-
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
46000
-
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
185000
-
gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
Pyrococcus furiosus
-
H+ + reduced ferredoxin
-
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
Pyrococcus furiosus
ferredoxin acts as physiological electron donor
H+ + reduced acceptor
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.12.7.2
Pyrococcus furiosus
-
strain DSM 3638
-
1.12.99.6
Pyrococcus furiosus
-
strain DSM 3638
-
Purification (Commentary)
EC Number
Commentary
Organism
1.12.7.2
-
Pyrococcus furiosus
1.12.99.6
apparent homogeneity
Pyrococcus furiosus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.99.6
360
-
-
Pyrococcus furiosus
Storage Stability
EC Number
Storage Stability
Organism
1.12.99.6
-196°C no loss of activity after freezing and thawing under argon
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439632
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439632
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + oxidized ferredoxin
-
439632
Pyrococcus furiosus
H+ + reduced ferredoxin
-
439632
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
methyl viologen acts as electron acceptor
439632
Pyrococcus furiosus
H+ + reduced acceptor
-
439632
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
methylene blue acts as electron acceptor
439632
Pyrococcus furiosus
H+ + reduced acceptor
-
439632
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
reduced ferredoxin can act as acceptor
439632
Pyrococcus furiosus
H+ + reduced acceptor
-
439632
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
ferredoxin acts as physiological electron donor
439632
Pyrococcus furiosus
H+ + reduced acceptor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.12.7.2
hexamer
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
hexamer
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.12.7.2
95
-
above 95°C, hydrogen oxidation; hydrogen evolution
Pyrococcus furiosus
1.12.99.6
95
-
above
Pyrococcus furiosus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.12.7.2
100
-
most of activity is retained after 1 h incubation
Pyrococcus furiosus
1.12.99.6
23
-
no loss of activity after 2 months under anaerobic conditions
Pyrococcus furiosus
1.12.99.6
80
-
50% loss of activity after 23 h
Pyrococcus furiosus
1.12.99.6
90
-
50% loss of activity after 3 h
Pyrococcus furiosus
1.12.99.6
100
-
50% loss of activity after 2 h
Pyrococcus furiosus
1.12.99.6
105
-
50% loss of activity after 5 min
Pyrococcus furiosus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.12.7.2
O2
reversible inhibition
Pyrococcus furiosus
1.12.99.6
O2
reversible
Pyrococcus furiosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.12.99.6
0.08
-
H2
with methylene blue as electron acceptor
Pyrococcus furiosus
1.12.99.6
1.9
-
H2
with methyl viologen as electron acceptor
Pyrococcus furiosus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.12.99.6
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.12.7.2
Fe
31 atoms per mol
Pyrococcus furiosus
1.12.7.2
Ni
0.98 atoms per mol enzyme
Pyrococcus furiosus
1.12.99.6
Fe
31 g iron per 185 g enzyme
Pyrococcus furiosus
1.12.99.6
Ni
31 g nickel per 185 g enzyme
Pyrococcus furiosus
1.12.99.6
Sulfide
24 g acid labile sulfide per 185 g enzyme
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.12.7.2
24000
-
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
27000
-
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
46000
-
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.7.2
185000
-
gel filtration
Pyrococcus furiosus
1.12.99.6
24000
-
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
27000
-
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
46000
-
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
185000
-
gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.12.7.2
H2 + oxidized ferredoxin
Pyrococcus furiosus
-
H+ + reduced ferredoxin
-
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
Pyrococcus furiosus
ferredoxin acts as physiological electron donor
H+ + reduced acceptor
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.12.7.2
-
Pyrococcus furiosus
1.12.99.6
apparent homogeneity
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.12.99.6
360
-
-
Pyrococcus furiosus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.12.99.6
-196°C no loss of activity after freezing and thawing under argon
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.12.7.2
H2 + electron acceptor
methylene blue as electron acceptor
439632
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + electron acceptor
methyl viologen as electron acceptor
439632
Pyrococcus furiosus
H+ + reduced electron acceptor
-
-
-
r
1.12.7.2
H2 + oxidized ferredoxin
-
439632
Pyrococcus furiosus
H+ + reduced ferredoxin
-
439632
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
methyl viologen acts as electron acceptor
439632
Pyrococcus furiosus
H+ + reduced acceptor
-
439632
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
methylene blue acts as electron acceptor
439632
Pyrococcus furiosus
H+ + reduced acceptor
-
439632
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
reduced ferredoxin can act as acceptor
439632
Pyrococcus furiosus
H+ + reduced acceptor
-
439632
Pyrococcus furiosus
r
1.12.99.6
H2 + acceptor
ferredoxin acts as physiological electron donor
439632
Pyrococcus furiosus
H+ + reduced acceptor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.12.7.2
hexamer
alpha, beta, gamma, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
1.12.99.6
hexamer
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.12.7.2
95
-
above 95°C, hydrogen oxidation; hydrogen evolution
Pyrococcus furiosus
1.12.99.6
95
-
above
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.12.7.2
100
-
most of activity is retained after 1 h incubation
Pyrococcus furiosus
1.12.99.6
23
-
no loss of activity after 2 months under anaerobic conditions
Pyrococcus furiosus
1.12.99.6
80
-
50% loss of activity after 23 h
Pyrococcus furiosus
1.12.99.6
90
-
50% loss of activity after 3 h
Pyrococcus furiosus
1.12.99.6
100
-
50% loss of activity after 2 h
Pyrococcus furiosus
1.12.99.6
105
-
50% loss of activity after 5 min
Pyrococcus furiosus