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Literature summary extracted from

  • Wang, Y.Z.; Lipscomb, J.D.
    Cloning, overexpression, and mutagenesis of the gene for homoprotocatechuate 2,3-dioxygenase from Brevibacterium fuscum (1997), Protein Expr. Purif., 10, 1-9.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.15 overexpression in Escherichia coli Brevibacterium fuscum

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.15 additional information triple mutant in which the three gaps in the sequence are removed does not show a significant change in metal content Brevibacterium fuscum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.15 0.011
-
 3,4-Dihydroxyphenylacetate recombinant enzyme Brevibacterium fuscum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.15 Iron recombinant enzyme contains 2.8 mol of iron per mol of enzyme Brevibacterium fuscum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2 Brevibacterium fuscum key ring cleavage step in metabolism of 3,4-dihydroxyphenylacetate 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Brevibacterium fuscum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.15 6.2
-
 recombinant enzyme Brevibacterium fuscum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Brevibacterium fuscum 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2 key ring cleavage step in metabolism of 3,4-dihydroxyphenylacetate Brevibacterium fuscum 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.15 additional information
-
 additional information turnover-numbers of recombinant wild-type enzyme and mutant enzyme Brevibacterium fuscum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.15 8.5
-
 recombinant wild-type enzyme and mutant enzyme Brevibacterium fuscum