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Literature summary extracted from

  • Milo, R.E.; Duffner, F.M.; Muller, R.
    Catechol 2,3-dioxygenase from the thermophilic, phenol-degrading Bacillus thermoleovorans strain A2 has unexpected low thermal stability (1999), Extremophiles, 3, 185-190.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.2
-
 Escherichia coli
1.13.11.2
-
 Geobacillus thermoleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.2 Fe2+ one iron per enzyme subunit, AAS, iron is tightly bound Geobacillus thermoleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 34700
-
 4 * 34700, SDS-PAGE Geobacillus thermoleovorans
1.13.11.2 135000
-
 gel filtration Geobacillus thermoleovorans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.13.11.2 additional information Tris-HCl, inhibitory effect on the activity Geobacillus thermoleovorans

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Escherichia coli
-
-
-
1.13.11.2 Geobacillus thermoleovorans
-
-
-
1.13.11.2 Geobacillus thermoleovorans A2
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.2 addition of H2O2 to the enzyme completely destroyes activity Geobacillus thermoleovorans

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
 Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 additional information
-
-
 Geobacillus thermoleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-chlorocatechol + O2 weak Geobacillus thermoleovorans 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 3-chlorocatechol + O2 weak Geobacillus thermoleovorans A2 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Escherichia coli 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2 weak Geobacillus thermoleovorans 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2 weak Geobacillus thermoleovorans A2 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Escherichia coli 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 weak Geobacillus thermoleovorans 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 weak Geobacillus thermoleovorans A2 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Escherichia coli 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Geobacillus thermoleovorans 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Geobacillus thermoleovorans A2 2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 tetramer 4 * 34700, SDS-PAGE Geobacillus thermoleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 70
-
-
 Geobacillus thermoleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.2 70
-
 1.5 min, 50% loss of original activity under aerobic conditions Geobacillus thermoleovorans
1.13.11.2 70
-
 10 min, 50% loss of original activity under nitrogen atmosphere Geobacillus thermoleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 7.2
-
-
 Geobacillus thermoleovorans