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Literature summary extracted from

  • Sakakibara, S.; Yamaguchi, K.; Hosokawa, Y.; Kohashi, N.; Ueda, I.; Sakamoto, Y.
    Purification and some properties of rat liver cysteine oxidase (cysteine dioxygenase) (1976), Biochim. Biophys. Acta, 422, 273-279.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.20 2,2'-dipyridyl slight activation at 0.01 mM Rattus norvegicus
1.13.11.20 8-hydroxyquinoline slight activation at 0.01 mM Rattus norvegicus
1.13.11.20 L-cysteine activates the purified enzyme under anaerobic conditions Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.20 2,2'-dipyridyl 92% inhibition at 0.1 mM Rattus norvegicus
1.13.11.20 8-hydroxyquinoline 59% inhibition at 0.1 mM Rattus norvegicus
1.13.11.20 Bathocuproine sulfonate 80% inhibition at 0.01 mM, 83% inhibition at 0.1 mM Rattus norvegicus
1.13.11.20 bathophenanthroline sulfonate 68% inhibition at 0.01 mM, 100% inhibition at 0.1 mM Rattus norvegicus
1.13.11.20 Cu2+ 90% inhibition at 0.01 mM, 100% inhibition at 0.1 mM Rattus norvegicus
1.13.11.20 cystine 42% inhibition at 5 mM Rattus norvegicus
1.13.11.20 D-cysteine 20% inhibition at 10 mM Rattus norvegicus
1.13.11.20 diethyldithiocarbamate no significant inhibition Rattus norvegicus
1.13.11.20 DL-homocysteine 47% inhibition at 10 mM Rattus norvegicus
1.13.11.20 DL-homocystine 47% inhibition at 5 mM Rattus norvegicus
1.13.11.20 EDTA 59% inhibition at 0.01 mM, 100% inhibition at 0.1 mM Rattus norvegicus
1.13.11.20 Fe2+ 50% inhibition at 0.01 mM, 100% inhibition at 0.1 mM Rattus norvegicus
1.13.11.20 o-phenanthroline 13% inhibition at 0.01 mM, 88% inhibition at 0.1 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.20 1.3
-
 L-cysteine
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.20 Fe prosthetic group Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.20 Rattus norvegicus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.20 rapid and irreversible inactivation under aerobic conditions, inactivation can be prevented by a distinct cytoplasmic protein, i.e. protein A Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.20 using heat treatment, ammonium sulfate fractionation and column chromatography on DEAE-cellulose, Sephadex G-200 and Sephadex G-100 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.20 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.20 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.13.11.20 -20°C, 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.20 L-cysteine + O2 highly specific for L-cysteine Rattus norvegicus 3-sulfino-L-alanine
-
 ?
1.13.11.20 additional information L-cystine, D-cysteine, DL-homocysteine and cysteamine do not serve as substrates Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.20 38 40 for the anaerobic activation of the purified enzyme by L-cysteine Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.20 6.8 9.5 for the anaerobic activation of the purified enzyme by L-cysteine Rattus norvegicus
1.13.11.20 8.5 9 purified enzyme, of enzyme reaction Rattus norvegicus