EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.3 | expression of wild-type and Y447H mutant enzyme in Pseudomonas fluorescens | Pseudomonas putida |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.11.3 | vapor diffusion with ammonium sulfate as the precipitant at 4°C, recombinant wild-type and Y447H mutant enzyme | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.3 | Y447H | greatly reduced rate of protocatechuate oxygenation | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.3 | 0.0029 | - |
protocatechuate | Y447H mutant enzyme | Pseudomonas putida | |
1.13.11.3 | 0.006 | - |
O2 | Y447H mutant enzyme | Pseudomonas putida | |
1.13.11.3 | 0.029 | - |
protocatechuate | recombinant enzyme | Pseudomonas putida | |
1.13.11.3 | 0.03 | - |
protocatechuate | native enzyme | Pseudomonas putida | |
1.13.11.3 | 0.04 | - |
O2 | native enzyme | Pseudomonas putida | |
1.13.11.3 | 0.056 | - |
O2 | recombinant enzyme | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.3 | no activity in Pseudomonas fluorescens | - |
- |
- |
1.13.11.3 | Pseudomonas putida | - |
previously classified as Pseudomonas aeruginosa strain ATCC 23975 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.3 | ammonium sulfate, DEAE-Sepharose, octyl-Sepharose, Phenyl-sepharose, recombinant enzyme | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.3 | 37.2 | - |
recombinant enzyme | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.3 | 3,4-dihydroxybenzoate + O2 | - |
Pseudomonas putida | 3-carboxy-cis,cis-muconate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.3 | 0.12 | - |
protocatechuate | Y447H mutant enzyme | Pseudomonas putida | |
1.13.11.3 | 68 | - |
protocatechuate | recombinant enzyme | Pseudomonas putida | |
1.13.11.3 | 70 | - |
protocatechuate | native enzyme | Pseudomonas putida |