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Literature summary extracted from

  • Hammer, A.; Stolz, A.; Knackmuss, H.J.
    Purification and characterization of a novel type of protocatechuate 3,4-dioxygenase with the ability to oxidize 4-sulfocatechol (1996), Arch. Microbiol., 166, 92-100.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.3 4-sulfocatechol competitive vs. protocatechuate Agrobacterium tumefaciens
1.13.11.3 4-sulfocatechol competitive vs. protocatechuate Hydrogenophaga palleronii
1.13.11.3 protocatechuate competitive vs. 4-sulfocatechol Agrobacterium tumefaciens
1.13.11.3 protocatechuate competitive vs. 4-sulfocatechol Hydrogenophaga palleronii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.3 0.042
-
 4-sulfocatechol protocatechuate 3,4-dioxygenase type II Hydrogenophaga palleronii
1.13.11.3 0.054
-
 protocatechuate protocatechuate 3,4-dioxygenase type I Agrobacterium tumefaciens
1.13.11.3 0.058
-
 protocatechuate protocatechuate 3,4-dioxygenase type II Hydrogenophaga palleronii
1.13.11.3 0.058
-
 4-sulfocatechol protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens
1.13.11.3 0.205
-
 protocatechuate protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Agrobacterium tumefaciens
-
 protocatechuate 3,4-dioxygenase I and II
-
1.13.11.3 Agrobacterium tumefaciens S2
-
 protocatechuate 3,4-dioxygenase I and II
-
1.13.11.3 Hydrogenophaga palleronii
-
 protocatechuate 3,4-dioxygenase II
-
1.13.11.3 Hydrogenophaga palleronii S1
-
 protocatechuate 3,4-dioxygenase II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3 Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, phenyl-superose HR5/5, Mono Q, type II protocatechuate 3,4-dioxygenase Agrobacterium tumefaciens
1.13.11.3 Q-Sepharose FF, ammonium sulfate, Sephacryl S-300HR, Phenyl-Superose, type I protocatechuate 3,4-dioxygenase Agrobacterium tumefaciens
1.13.11.3 Q-Sepharose FF, ammonium sulfate, Superdex 200, Fractogel, type II protocatechuate 3,4-dioxygenase Hydrogenophaga palleronii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.3 5.2
-
 type II protocatechuate 3,4-dioxygenase, oxidation of 4-sulfocatechol Hydrogenophaga palleronii
1.13.11.3 16.7
-
 type II protocatechuate 3,4-dioxygenase, oxidation of 4-sulfocatechol Agrobacterium tumefaciens
1.13.11.3 105
-
 type I protocatechuate 3,4-dioxygenase Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Agrobacterium tumefaciens 3-carboxy-cis,cis-muconate
-
 ?
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Hydrogenophaga palleronii 3-carboxy-cis,cis-muconate
-
 ?
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Agrobacterium tumefaciens S2 3-carboxy-cis,cis-muconate
-
 ?
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Hydrogenophaga palleronii S1 3-carboxy-cis,cis-muconate
-
 ?
1.13.11.3 3,4-dihydroxymandelic acid + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii ?
-
 ?
1.13.11.3 3,4-dihydroxymandelic acid + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii S1 ?
-
 ?
1.13.11.3 3,4-dihydroxyphenylacetate + O2
-
 Agrobacterium tumefaciens ?
-
 ?
1.13.11.3 3,4-dihydroxyphenylacetate + O2
-
 Hydrogenophaga palleronii ?
-
 ?
1.13.11.3 3-(3,4-dihydroxyphenyl)propionate + O2 protocatechuate 3,4-dioxygenase II Hydrogenophaga palleronii ?
-
 ?
1.13.11.3 3-methylcatechol + O2
-
 Agrobacterium tumefaciens 2-methylmuconate
-
 ?
1.13.11.3 3-methylcatechol + O2
-
 Hydrogenophaga palleronii 2-methylmuconate
-
 ?
1.13.11.3 3-methylcatechol + O2
-
 Agrobacterium tumefaciens S2 2-methylmuconate
-
 ?
1.13.11.3 3-methylcatechol + O2
-
 Hydrogenophaga palleronii S1 2-methylmuconate
-
 ?
1.13.11.3 4-methylcatechol + O2 at very low rates Agrobacterium tumefaciens 3-methyl-cis,cis-muconate
-
 ?
1.13.11.3 4-methylcatechol + O2 at very low rates Hydrogenophaga palleronii 3-methyl-cis,cis-muconate
-
 ?
1.13.11.3 4-methylcatechol + O2 at very low rates Agrobacterium tumefaciens S2 3-methyl-cis,cis-muconate
-
 ?
1.13.11.3 4-methylcatechol + O2 at very low rates Hydrogenophaga palleronii S1 3-methyl-cis,cis-muconate
-
 ?
1.13.11.3 4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Agrobacterium tumefaciens 3-sulfomuconate
-
 ?
1.13.11.3 4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Hydrogenophaga palleronii 3-sulfomuconate
-
 ?
1.13.11.3 4-sulfocatechol + O2 catalyzed by protocatechuate 3,4-dioxygenase type II only Agrobacterium tumefaciens S2 3-sulfomuconate
-
 ?
1.13.11.3 catechol + O2
-
 Agrobacterium tumefaciens muconate
-
 ?
1.13.11.3 catechol + O2
-
 Hydrogenophaga palleronii muconate
-
 ?
1.13.11.3 catechol + O2
-
 Agrobacterium tumefaciens S2 muconate
-
 ?
1.13.11.3 catechol + O2
-
 Hydrogenophaga palleronii S1 muconate
-
 ?
1.13.11.3 trans-3,4-dihydroxycinnamate + O2
-
 Agrobacterium tumefaciens ?
-
 ?
1.13.11.3 trans-3,4-dihydroxycinnamate + O2
-
 Hydrogenophaga palleronii ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.3 14.6
-
 4-sulfocatechol protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens
1.13.11.3 18.8
-
 protocatechuate protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens
1.13.11.3 89.5
-
 protocatechuate protocatechuate 3,4-dioxygenase type I Agrobacterium tumefaciens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.3 0.032
-
 4-sulfocatechol protocatechuate 3,4-dioxygenase type II Hydrogenophaga palleronii
1.13.11.3 0.053
-
 4-sulfocatechol protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens
1.13.11.3 0.066
-
 protocatechuate protocatechuate 3,4-dioxygenase type II Hydrogenophaga palleronii
1.13.11.3 0.102
-
 protocatechuate protocatechuate 3,4-dioxygenase type II Agrobacterium tumefaciens