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Literature summary extracted from

  • Durham, D.R.; Stirling, L.A.; Ornsten, L.N.; Perry, J.J.
    Intergeneric evolutionary homology revealed by the study of protocatechuate 3,4-dioxygenase from Azotobacter vinelandii (1980), Biochemistry, 19, 149-155.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.3 2,3-Dihydroxybenzoate
-
 Azotobacter vinelandii
1.13.11.3 3,4-dihydroxyphenylacetic acid
-
 Azotobacter vinelandii
1.13.11.3 4-(dimethylamino)benzaldehyde
-
 Azotobacter vinelandii
1.13.11.3 4-hydroxybenzoate
-
 Azotobacter vinelandii
1.13.11.3 4-methylcatechol
-
 Azotobacter vinelandii
1.13.11.3 4-nitrocatechol
-
 Azotobacter vinelandii
1.13.11.3 caffeic acid
-
 Azotobacter vinelandii
1.13.11.3 catechol
-
 Azotobacter vinelandii
1.13.11.3 Protocatechualdehyde
-
 Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.3 0.018
-
 protocatechuate
-
 Azotobacter vinelandii
1.13.11.3 0.0275
-
 O2
-
 Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.3 Fe 10 g-atoms per mol enzyme Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.3 23300
-
 alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE Azotobacter vinelandii
1.13.11.3 25500
-
 alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE Azotobacter vinelandii
1.13.11.3 480000
-
 ultracentrifugation Azotobacter vinelandii
1.13.11.3 510000
-
 gel filtration Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Azotobacter vinelandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3 ammonium sulfate, DEAE-cellulose, Bio-gel agarose, DEAE-cellulose Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.3 46.69
-
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2
-
 Azotobacter vinelandii 3-carboxy-cis,cis-muconate
-
 ?
1.13.11.3 3-methylcatechol + O2 at 5% the rate of protocatechuic acid oxidation Azotobacter vinelandii 2-methylmuconate
-
 ?
1.13.11.3 catechol + O2 at 3% the rate of protocatechuic acid oxidation Azotobacter vinelandii muconate
-
 ?
1.13.11.3 pyrogallol + O2 at 10% the rate of protocatechuic acid oxidation Azotobacter vinelandii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.3 decamer alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE Azotobacter vinelandii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.3 50
-
 pH 8.5, 90 min, stable Azotobacter vinelandii
1.13.11.3 60
-
 pH 8.5, 10 min, 90% loss of activity Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.3 8.5
-
-
 Azotobacter vinelandii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.3 8.5
-
 50°C, 90 min stable Azotobacter vinelandii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.3 0.0006
-
 4-nitrocatechol
-
 Azotobacter vinelandii
1.13.11.3 0.001
-
 Protocatechualdehyde
-
 Azotobacter vinelandii