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Literature summary extracted from

  • Stanier, R.Y.; Ingraham, J.L.
    Protocatechuic acid oxidase (1954), J. Biol. Chem., 210, 799-808.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.3 iodoacetate 5 mM, 20% inhibition Pseudomonas fluorescens
1.13.11.3 KF 50 mM, 34% inhibition Pseudomonas fluorescens
1.13.11.3 p-chloromercuribenzoate 0.05 mM, 47% inhibition Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.3 Pseudomonas fluorescens
-
-
-
1.13.11.3 Pseudomonas fluorescens A.3.12.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.3 partial Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.3 33.45
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.13.11.3 0-3°C, several weeks, no loss in activity Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.3 3,4-dihydroxybenzoate + O2 no other substrate found Pseudomonas fluorescens 3-carboxy-cis,cis-muconate
-
 ir
1.13.11.3 3,4-dihydroxybenzoate + O2 no other substrate found Pseudomonas fluorescens A.3.12. 3-carboxy-cis,cis-muconate
-
 ir

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.3 additional information
-
 activity increases steadily up to pH 9.0, above pH 9.0 the enzyme begins to undergo spontaneous oxidation Pseudomonas fluorescens