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Literature summary extracted from

  • Zhang, Y.Y.; Lind, B.; Raadmark, O.; Samuelsson, B.
    Iron content of human 5-lipoxygenase. Effects of mutations regarding conserved histidine residues (1993), J. Biol. Chem., 268, 2535-2541.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.34 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.34 H362Q reduced enzyme activity, similar amounts of iron as non-mutated enzyme Homo sapiens
1.13.11.34 H367N inactive, contains 0.2 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme Homo sapiens
1.13.11.34 H367Q inactive, contains 0.5 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme Homo sapiens
1.13.11.34 H367S inactive, contains 0.5 mol of iron per mol of protein, compared to 0.86 mol of iron per mol of protein for the wild-type enzyme Homo sapiens
1.13.11.34 H372Q no detectable enzyme activity, practically devoid of iron Homo sapiens
1.13.11.34 H390Q reduced enzyme activity, similar amounts of iron as non-mutated enzyme Homo sapiens
1.13.11.34 H399Q reduced enzyme activity, similar amounts of iron as non-mutated enzyme Homo sapiens
1.13.11.34 H550Q no detectable enzyme activity, practically devoid of iron Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Iron wild-type enzyme contains 0.65 mol of iron per mol of enzyme, H372 and H550 constitute two of the iron ligands Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.34 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.13.11.34 -20°C, stable for weeks Homo sapiens
1.13.11.34 4°C, stable for days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Homo sapiens (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate
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