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Literature summary extracted from

  • Mulliez, E.; Leblanc, J.P.; Girerd, J.J.; Rigaud, M.; Chottard, J.C.
    5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics (1987), Biochim. Biophys. Acta, 916, 13-23.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.13.11.34 efficiently stabilized by 20% v/v glycerol Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.34 acetonylacetone bisphenylhydrazone IC50 for reaction with linoleic acid: 0.015 mM, IC50 for reaction with arachidonic acid: 0.0039 mM Solanum tuberosum
1.13.11.34 benzaldehyde phenylhydrazone IC50 for reaction with linoleic acid: 0.0345 mM, IC50 for reaction with arachidonic acid: 0.0014 mM Solanum tuberosum
1.13.11.34 BW755C CAS: 66000-40-6 Solanum tuberosum
1.13.11.34 nordihydroguiaraetic acid IC50 for reaction with linoleic acid: 0.17 mM, IC50 for reaction with arachidonic acid: 0.0365 mM Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.34 0.038
-
 arachidonic acid
-
 Solanum tuberosum
1.13.11.34 0.039
-
 linoleic acid
-
 Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.34 Iron contains one iron atom per molecule, the native enzyme is in a pseudo-axial high-spin ferric state Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.34 92000
-
 x * 92000, SDS-PAGE Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.34 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.34 major isoenzyme L1 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.34 tuber contains at least 3 isoenzymes Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.34 147
-
-
 Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.34 arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Solanum tuberosum (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate
-
 ?
1.13.11.34 linoleic acid + O2
-
 Solanum tuberosum 9-hydroperoxyoctadecadienoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.34 ? x * 92000, SDS-PAGE Solanum tuberosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.34 25
-
 arachidonic acid
-
 Solanum tuberosum
1.13.11.34 138
-
 linoleic acid
-
 Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.34 5.5
-
-
 Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.34 3.5 7 almost inactive at pH 3.5 and at pH 7.0 Solanum tuberosum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.34 0.0014
-
 IC50 for reaction with linoleic acid: 0.0345 mM, IC50 for reaction with arachidonic acid: 0.0014 mM Solanum tuberosum benzaldehyde phenylhydrazone
1.13.11.34 0.0039
-
 IC50 for reaction with linoleic acid: 0.015 mM, IC50 for reaction with arachidonic acid: 0.0039 mM Solanum tuberosum acetonylacetone bisphenylhydrazone
1.13.11.34 0.0365
-
 IC50 for reaction with linoleic acid: 0.17 mM, IC50 for reaction with arachidonic acid: 0.0365 mM Solanum tuberosum nordihydroguiaraetic acid