EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.34 | 0.03 | - |
arachidonic acid | - |
Solanum tuberosum | |
1.13.11.34 | 0.033 | - |
arachidonic acid | - |
Solanum tuberosum | |
1.13.11.34 | 0.05 | - |
bishomo-gamma-linolenic acid | - |
Solanum tuberosum | |
1.13.11.34 | 0.2 | - |
linoleic acid | - |
Solanum tuberosum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.34 | 35000 | - |
x * 35000, enzyme form L2, SDS-PAGE | Solanum tuberosum |
1.13.11.34 | 85000 | - |
enzyme form L1 and L2, gel filtration | Solanum tuberosum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.34 | Solanum tuberosum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.34 | - |
Solanum tuberosum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.34 | tuber | - |
Solanum tuberosum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.34 | 5.7 | - |
enzyme form L2 | Solanum tuberosum |
1.13.11.34 | 13.7 | - |
enzyme form L1 | Solanum tuberosum |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.34 | -20°C, 40 mM potassium phosphate buffer, pH 6.3, 2 M (NH4)2SO4, enzyme form L1 is stable for months | Solanum tuberosum |
1.13.11.34 | 4°C, the L1 form is gradually transformed into the L2 form with a significant reduction in specific activity, the L2 form loses all of its activity over a few days and more rapid in absence of (NH4)2SO4 | Solanum tuberosum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.34 | arachidonate + O2 | i.e. 5,8,11,14-eicosatetraenoic acid | Solanum tuberosum | (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate | 5(S)-hydroperoxyeicosatetraenoic acid is the most abundant primary oxygenation product, representing 60% of the total hydroperoxyeicosatetraenoic acids, followed by 8(S)-hydroperoxyeicosatetraenoic acid, 9-hydroperoxyeicosatetraenoic acid, 11(S)-hydroperoxyeicosatetraenoic acid, 12-hydroperoxyeicosatetraenoic acid, and 15-hydroperoxyeicosatetraenoic acid in decreasing order of abundance, all possible diH(P)ETEs are detected | ? | |
1.13.11.34 | bishomo-gamma-linolenic acid + O2 | - |
Solanum tuberosum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.34 | ? | x * 35000, enzyme form L2, SDS-PAGE | Solanum tuberosum |
1.13.11.34 | ? | x * 85000, enzyme form L2, SDS-PAGE | Solanum tuberosum |
1.13.11.34 | More | the L1 form is gradually transformed into the L2 form with a significant reduction in specific activity | Solanum tuberosum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.34 | additional information | - |
pH of the reaction has profound influence on the product profile | Solanum tuberosum |