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Inhibition analysis of hydroxyquinol-cleaving dioxygenases from the chlorophenol-degrading Azotobacter sp. GP1 and Streptomyces rochei 303

Zaborina, O.; Seitz, H.J.; Sidorov, I.; Eberspaecher, J.; Alexeeva, E.; Golovleva, L.; Lingens, F.; J. Basic Microbiol. 39, 61-73 (1999)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.11.37
Fe(NH4)2(SO4)2
no activation by Fe2+ ions
Azotobacter sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.37
2,4-Dichlorophenol
mixed inhibition
Azotobacter sp.
1.13.11.37
2,4-Dichlorophenol
competitive inhibition
Streptomyces rochei
1.13.11.37
2,6-dichlorohydroquinone
competitive nhibition
Azotobacter sp.
1.13.11.37
2,6-dichlorohydroquinone
competitive inhibition
Streptomyces rochei
1.13.11.37
3,4,6-trichlorocatechol
mixed inhibition
Azotobacter sp.
1.13.11.37
3,4,6-trichlorocatechol
mixed inhibition
Streptomyces rochei
1.13.11.37
3,5-dichlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
3,5-dichlorocatechol
mixed inhibition
Streptomyces rochei
1.13.11.37
3-chlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
3-chlorocatechol
mixd inhibition
Streptomyces rochei
1.13.11.37
3-methyl-5-chlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
3-methyl-5-chlorocatechol
mixed inhibition
Streptomyces rochei
1.13.11.37
4,5-dichlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
4,5-dichlorocatechol
parially mixed competitive inhibition
Streptomyces rochei
1.13.11.37
4-Chlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
4-Chlorocatechol
competitive inhibition
Streptomyces rochei
1.13.11.37
catechol
mixed inhibition
Azotobacter sp.
1.13.11.37
EDTA
slight inhibition
Azotobacter sp.
1.13.11.37
monochlorohydroquinone
competitive inhibition
Azotobacter sp.
1.13.11.37
monochlorohydroquinone
competitive inhibition
Streptomyces rochei
1.13.11.37
pyrogallol
mixed inhibition
Azotobacter sp.
1.13.11.37
tetrachlorocatechol
mixed inhibition
Azotobacter sp.
1.13.11.37
tetrachlorocatechol
mixed inhibition
Streptomyces rochei
1.13.11.37
tetrachlorohydroquinone
competitive inhibition
Azotobacter sp.
1.13.11.37
tetrachlorohydroquinone
competitive inhibition
Streptomyces rochei
1.13.11.37
Tiron
-
Azotobacter sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.37
Iron
1 mol of iron per mol enzyme
Azotobacter sp.
1.13.11.37
Iron
1 mol of iron per mol enzyme
Streptomyces rochei
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.37
Azotobacter sp.
-
grown with 2,4,6-trichlorophenol
-
1.13.11.37
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
-
grown with 2,4,6-trichlorophenol
-
1.13.11.37
Streptomyces rochei
-
strain 303 VKM AC-1284D, grown with 2,4,6-trichlorophenol
-
1.13.11.37
Streptomyces rochei DSM 40242
-
strain 303 VKM AC-1284D, grown with 2,4,6-trichlorophenol
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.37
-
Azotobacter sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.13.11.37
additional information
-
-
Azotobacter sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.13.11.37
0.00069
-
pyrogallol
-
Azotobacter sp.
1.13.11.37
0.00083
-
monochlorohydroquinone
-
Streptomyces rochei
1.13.11.37
0.00107
-
monochlorohydroquinone
-
Azotobacter sp.
1.13.11.37
0.00128
-
tetrachlorocatechol
-
Streptomyces rochei
1.13.11.37
0.00226
-
tetrachlorohydroquinone
-
Streptomyces rochei
1.13.11.37
0.00349
-
tetrachlorocatechol
-
Azotobacter sp.
1.13.11.37
0.00536
-
3,4,6-trichlorocatechol
-
Streptomyces rochei
1.13.11.37
0.00585
-
4,5-dichlorocatechol
-
Streptomyces rochei
1.13.11.37
0.00589
-
2,6-dichlorohydroquinone
-
Streptomyces rochei
1.13.11.37
0.00698
-
3-chlorocatechol
-
Azotobacter sp.
1.13.11.37
0.0073
-
4-Chlorocatechol
-
Azotobacter sp.
1.13.11.37
0.0088
-
3,5-dichlorocatechol
-
Azotobacter sp.
1.13.11.37
0.00956
-
catechol
-
Azotobacter sp.
1.13.11.37
0.0103
-
4,5-dichlorocatechol
-
Azotobacter sp.
1.13.11.37
0.01094
-
2,6-dichlorohydroquinone
-
Azotobacter sp.
1.13.11.37
0.0112
-
3,4,6-trichlorocatechol
-
Azotobacter sp.
1.13.11.37
0.01248
-
tetrachlorohydroquinone
-
Azotobacter sp.
1.13.11.37
0.01274
-
2,4-Dichlorophenol
-
Azotobacter sp.
1.13.11.37
0.0169
-
3,5-dichlorocatechol
-
Streptomyces rochei
1.13.11.37
0.01719
-
2,4-Dichlorophenol
-
Streptomyces rochei
1.13.11.37
0.02732
-
3-methyl-5-chlorocatechol
-
Azotobacter sp.
1.13.11.37
0.03077
-
3-chlorocatechol
-
Streptomyces rochei
1.13.11.37
0.0373
-
3-methyl-5-chlorocatechol
-
Streptomyces rochei
1.13.11.37
0.0526
-
4-Chlorocatechol
-
Streptomyces rochei
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.13.11.37
Fe(NH4)2(SO4)2
no activation by Fe2+ ions
Azotobacter sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.37
2,4-Dichlorophenol
mixed inhibition
Azotobacter sp.
1.13.11.37
2,4-Dichlorophenol
competitive inhibition
Streptomyces rochei
1.13.11.37
2,6-dichlorohydroquinone
competitive nhibition
Azotobacter sp.
1.13.11.37
2,6-dichlorohydroquinone
competitive inhibition
Streptomyces rochei
1.13.11.37
3,4,6-trichlorocatechol
mixed inhibition
Azotobacter sp.
1.13.11.37
3,4,6-trichlorocatechol
mixed inhibition
Streptomyces rochei
1.13.11.37
3,5-dichlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
3,5-dichlorocatechol
mixed inhibition
Streptomyces rochei
1.13.11.37
3-chlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
3-chlorocatechol
mixd inhibition
Streptomyces rochei
1.13.11.37
3-methyl-5-chlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
3-methyl-5-chlorocatechol
mixed inhibition
Streptomyces rochei
1.13.11.37
4,5-dichlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
4,5-dichlorocatechol
parially mixed competitive inhibition
Streptomyces rochei
1.13.11.37
4-Chlorocatechol
competitive inhibition
Azotobacter sp.
1.13.11.37
4-Chlorocatechol
competitive inhibition
Streptomyces rochei
1.13.11.37
catechol
mixed inhibition
Azotobacter sp.
1.13.11.37
EDTA
slight inhibition
Azotobacter sp.
1.13.11.37
monochlorohydroquinone
competitive inhibition
Azotobacter sp.
1.13.11.37
monochlorohydroquinone
competitive inhibition
Streptomyces rochei
1.13.11.37
pyrogallol
mixed inhibition
Azotobacter sp.
1.13.11.37
tetrachlorocatechol
mixed inhibition
Azotobacter sp.
1.13.11.37
tetrachlorocatechol
mixed inhibition
Streptomyces rochei
1.13.11.37
tetrachlorohydroquinone
competitive inhibition
Azotobacter sp.
1.13.11.37
tetrachlorohydroquinone
competitive inhibition
Streptomyces rochei
1.13.11.37
Tiron
-
Azotobacter sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.13.11.37
0.00069
-
pyrogallol
-
Azotobacter sp.
1.13.11.37
0.00083
-
monochlorohydroquinone
-
Streptomyces rochei
1.13.11.37
0.00107
-
monochlorohydroquinone
-
Azotobacter sp.
1.13.11.37
0.00128
-
tetrachlorocatechol
-
Streptomyces rochei
1.13.11.37
0.00226
-
tetrachlorohydroquinone
-
Streptomyces rochei
1.13.11.37
0.00349
-
tetrachlorocatechol
-
Azotobacter sp.
1.13.11.37
0.00536
-
3,4,6-trichlorocatechol
-
Streptomyces rochei
1.13.11.37
0.00585
-
4,5-dichlorocatechol
-
Streptomyces rochei
1.13.11.37
0.00589
-
2,6-dichlorohydroquinone
-
Streptomyces rochei
1.13.11.37
0.00698
-
3-chlorocatechol
-
Azotobacter sp.
1.13.11.37
0.0073
-
4-Chlorocatechol
-
Azotobacter sp.
1.13.11.37
0.0088
-
3,5-dichlorocatechol
-
Azotobacter sp.
1.13.11.37
0.00956
-
catechol
-
Azotobacter sp.
1.13.11.37
0.0103
-
4,5-dichlorocatechol
-
Azotobacter sp.
1.13.11.37
0.01094
-
2,6-dichlorohydroquinone
-
Azotobacter sp.
1.13.11.37
0.0112
-
3,4,6-trichlorocatechol
-
Azotobacter sp.
1.13.11.37
0.01248
-
tetrachlorohydroquinone
-
Azotobacter sp.
1.13.11.37
0.01274
-
2,4-Dichlorophenol
-
Azotobacter sp.
1.13.11.37
0.0169
-
3,5-dichlorocatechol
-
Streptomyces rochei
1.13.11.37
0.01719
-
2,4-Dichlorophenol
-
Streptomyces rochei
1.13.11.37
0.02732
-
3-methyl-5-chlorocatechol
-
Azotobacter sp.
1.13.11.37
0.03077
-
3-chlorocatechol
-
Streptomyces rochei
1.13.11.37
0.0373
-
3-methyl-5-chlorocatechol
-
Streptomyces rochei
1.13.11.37
0.0526
-
4-Chlorocatechol
-
Streptomyces rochei
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.37
Iron
1 mol of iron per mol enzyme
Azotobacter sp.
1.13.11.37
Iron
1 mol of iron per mol enzyme
Streptomyces rochei
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.37
-
Azotobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.13.11.37
additional information
-
-
Azotobacter sp.