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Literature summary extracted from

  • Riegert, U.; Heiss, G.; Kuhm, A.E.; Mueller, C.; Contzen, M.; Knackmuss, H.J.; Stolz, A.
    Catalytic properties of the 3-chlorocatechol-oxidizing 2,3-dihydroxybiphenyl 1,2-dioxygenase from Sphingomonas sp. strain BN6 (1999), J. Bacteriol., 181, 4812-4817.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 genes BphC1 and BphC2 cloned and expressed in Escherichia coli JM108 and JM109 Sphingomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.39 3-chlorocatechol competitive inhibition Sphingomonas sp.
1.13.11.39 o-phenanthroline
-
 Sphingomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.003
-
 2,3-Dihydroxybiphenyl in presence of 3-chlorocatechol Sphingomonas sp.
1.13.11.39 0.007
-
 3,5-dichlorocatechol Glu79His mutant Sphingomonas sp.
1.13.11.39 0.025
-
 3-chlorocatechol Glu79His mutant Sphingomonas sp.
1.13.11.39 0.03
-
 3,5-dichlorocatechol
-
 Sphingomonas sp.
1.13.11.39 0.032
-
 2,3-Dihydroxybiphenyl Glu79His mutant Sphingomonas sp.
1.13.11.39 0.097
-
 3-methylcatechol Glu79His mutant Sphingomonas sp.
1.13.11.39 0.1
-
 3-chlorocatechol
-
 Sphingomonas sp.
1.13.11.39 0.18
-
 3-methylcatechol
-
 Sphingomonas sp.
1.13.11.39 0.67
-
 catechol Glu79His mutant Sphingomonas sp.
1.13.11.39 0.67
-
 catechol mutant enzyme E79H, at pH 7.5, temperature not specified in the publication Sphingomonas sp.
1.13.11.39 0.74
-
 2,3-Dihydroxybiphenyl
-
 Sphingomonas sp.
1.13.11.39 1.13
-
 catechol wild type enzyme, at pH 7.5, temperature not specified in the publication Sphingomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Fe2+ cofactor in the catalytic center Sphingomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2 Sphingomonas sp.
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Rhodococcus globerulus
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Sphingomonas sp. BN6
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Rhodococcus globerulus P6
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Rhodococcus globerulus
-
-
-
1.13.11.39 Rhodococcus globerulus P6
-
-
-
1.13.11.39 Sphingomonas sp.
-
-
-
1.13.11.39 Sphingomonas sp. BN6
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 3.1
-
 BphC1 Sphingomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 3,5-dichlorocatechol + O2
-
 Sphingomonas sp. 3,5-dichloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.39 3,5-dichlorocatechol + O2
-
 Sphingomonas sp. BN6 3,5-dichloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.39 3-chlorocatechol + O2
-
 Sphingomonas sp. 3-chloro-2-hydroxymuconic semialdehyde + H2O
-
 ?
1.13.11.39 3-chlorocatechol + O2
-
 Sphingomonas sp. BN6 3-chloro-2-hydroxymuconic semialdehyde + H2O
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Sphingomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Sphingomonas sp. BN6 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Sphingomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Sphingomonas sp. BN6 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Rhodococcus globerulus P6 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 catechol + O2
-
 Sphingomonas sp. cis,cis-muconate
-
 r
1.13.11.39 catechol + O2
-
 Sphingomonas sp. BN6 cis,cis-muconate
-
 r
1.13.11.39 catechol + O2
-
 Sphingomonas sp. 2-hydroxymuconate semialdehyde
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 BphC1-BN6
-
 Sphingomonas sp.
1.13.11.39 BphC2-BN6
-
 Sphingomonas sp.