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Literature summary extracted from

  • Vaillancourt, F.H.; Han, S.; Fortin, P.D.; Bolin, J.T.; Eltis, L.D.
    Molecular basis for the stabilization and inhibition of 2,3-dihydroxybiphenyl 1,2-dioxygenase by t-butanol (1998), J. Biol. Chem., 273, 34887-34895.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.39
-
 Burkholderia cepacia
1.13.11.39 strain KKS102 Pseudomonas sp.

General Stability

EC Number General Stability Organism
1.13.11.39 tert-butanol, glycerol, isopropylalcohol and ethanol stabilizes the enzyme Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.39 2,3-Dihydroxybiphenyl reversible substrate inhibition Burkholderia cepacia
1.13.11.39 2,3-Dihydroxybiphenyl
-
 Pseudomonas sp.
1.13.11.39 3-chlorocatechol
-
 Burkholderia cepacia
1.13.11.39 3-ethylcatechol reversible substrate inhibition Burkholderia cepacia
1.13.11.39 acetone competitive inhibition Burkholderia cepacia
1.13.11.39 ethanol
-
 Burkholderia cepacia
1.13.11.39 glycerol
-
 Burkholderia cepacia
1.13.11.39 isopropylalcohol
-
 Burkholderia cepacia
1.13.11.39 tert-butanol
-
 Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.022
-
 2,3-Dihydroxybiphenyl
-
 Burkholderia cepacia
1.13.11.39 1.28
-
 O2
-
 Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2 Pseudomonas sp.
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Burkholderia cepacia
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Burkholderia cepacia LB400
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Burkholderia cepacia
-
-
-
1.13.11.39 Burkholderia cepacia LB400
-
-
-
1.13.11.39 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.39
-
 Burkholderia cepacia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 431
-
-
 Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 3-ethylcatechol + O2
-
 Burkholderia cepacia 2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate
-
 ?
1.13.11.39 3-ethylcatechol + O2
-
 Burkholderia cepacia LB400 2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Burkholderia cepacia LB400 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Burkholderia cepacia LB400 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 catechol + O2
-
 Pseudomonas sp. ?
-
 ?
1.13.11.39 catechol + O2
-
 Burkholderia cepacia ?
-
 ?
1.13.11.39 catechol + O2
-
 Burkholderia cepacia LB400 ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.39 0.22
-
 3-chlorocatechol
-
 Burkholderia cepacia
1.13.11.39 3
-
 2,3-Dihydroxybiphenyl reversible substrate inhibition Burkholderia cepacia
1.13.11.39 5.2
-
 tert-butanol catechol as substrate Burkholderia cepacia
1.13.11.39 7.3
-
 tert-butanol 2,3-dihydroxybiphenyl as substrate Burkholderia cepacia
1.13.11.39 9.9
-
 tert-butanol 3-methylcatechol as substrate Burkholderia cepacia
1.13.11.39 10.5
-
 isopropylalcohol
-
 Burkholderia cepacia
1.13.11.39 11
-
 3-ethylcatechol
-
 Burkholderia cepacia
1.13.11.39 13
-
 acetone
-
 Burkholderia cepacia
1.13.11.39 35
-
 ethanol
-
 Burkholderia cepacia