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Literature summary extracted from

  • Eltis, L.D.; Hofmann, B.; Hecht, H.J.; Luensdorf, H.; Timmis, K.N.
    Purification and crystallization of 2,3-dihydroxybiphenyl 1,2-dioxygenase (1993), J. Biol. Chem., 268, 2727-2732.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39
-
 Pseudomonas sp.
1.13.11.39 bhpC gene hyperexpressed in strain LB400 with pLEBD4 Burkholderia cepacia

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.39 crystals grown from ammoinum sulfate and polyethylene glycol 6000, space groups of P42212 and I222 Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.39 2,3-Dihydroxybiphenyl
-
 Burkholderia cepacia
1.13.11.39 2,3-Dihydroxybiphenyl enzyme ist strongly inhibited by substrate, Kss 0.0003 M Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.007
-
 2,3-Dihydroxybiphenyl
-
 Pseudomonas sp.
1.13.11.39 0.19
-
 3-methylcatechol
-
 Pseudomonas sp.
1.13.11.39 0.54
-
 catechol
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.39 33200
-
 8 * 33200, SDS-PAGE Pseudomonas sp.
1.13.11.39 265000
-
 electron microscopy Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2 Burkholderia cepacia
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Pseudomonas sp. aerobic biphenyl biodegradation pathway 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Rhodococcus globerulus aerobic biphenyl biodegradation pathway 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Burkholderia cepacia
-
 organism reclassified, prior refered as Pseudomonas sp. strain LB400 or Pseudomonas cepacia strain LB400
-
1.13.11.39 Pseudomonas sp.
-
-
-
1.13.11.39 Rhodococcus globerulus
-
 formerly Acinetobacter sp. strain P6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.39
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 191
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 3-methylcatechol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 3-methylcatechol + O2 cleaved less efficiently, weaker substrate inhibition Pseudomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 4-methylcatechol + O2 cleaved less efficiently, weaker substrate inhibition Pseudomonas sp. 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Burkholderia cepacia 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 aerobic biphenyl biodegradation pathway Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 aerobic biphenyl biodegradation pathway Rhodococcus globerulus 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 catechol + O2
-
 Burkholderia cepacia ?
-
 ?
1.13.11.39 catechol + O2 cleaved less efficiently, weaker substrate inhibition Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.39 octamer 8 * 33200, SDS-PAGE Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.39 7 9
-
 Pseudomonas sp.