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Leukotriene A synthase activity of purified mouse skin arachidonate 8-lipoxygenase expressed in Escherichia coli

Qiao, N.; Takahashi, Y.; Takamatsu, H.; Yoshimoto, T.; Biochim. Biophys. Acta 1438, 131-139 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.40
skin 8-lipoxygenase expressed in COS-7 cells by transient transfection of its cDNA, amplified by PCR, in pEF-BOS, also expressed in Escherichia coli system using pQE-32
Mus musculus
General Stability
EC Number
General Stability
Organism
1.13.11.40
leukotriene A synthase activity of the enzyme leveled off within 10 min, indicating suicide activation
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.40
AA861
-
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.40
2 arachidonic acid + 2 O2
Mus musculus
-
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.40
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.40
recombinant enzyme
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.40
skin
-
Mus musculus
-
Storage Stability
EC Number
Storage Stability
Organism
1.13.11.40
-70°C, crude and purified enzyme preparations can be stored without appreciable loss of activity for at least 6 months
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.40
2 arachidonic acid + 2 O2
-
439401
Mus musculus
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
-
-
?
1.13.11.40
5-hydroperoxy fatty acid + O2
leukotriene A synthase activity, reaction rate is approximately 7% of arachidonate 8-lipoxygenation
439401
Mus musculus
leukotriene A4
-
-
-
?
1.13.11.40
5-hydroperoxyeicosatetraenoic acid + ?
-
439401
Mus musculus
leukotriene A4
unstable product
439401
Mus musculus
?
1.13.11.40
alpha-linolenic acid + O2
-
439401
Mus musculus
?
-
-
-
?
1.13.11.40
arachidonic acid + O2
-
439401
Mus musculus
(8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
-
-
?
1.13.11.40
docosahexaenoic acid + O2
-
439401
Mus musculus
?
-
-
-
?
1.13.11.40
eicosapentaenoic acid + O2
-
439401
Mus musculus
?
-
-
-
?
1.13.11.40
gamma-linoleic acid + O2
-
439401
Mus musculus
?
-
-
-
?
1.13.11.40
linoleic acid + O2
poor substrate
439401
Mus musculus
?
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.40
skin 8-lipoxygenase expressed in COS-7 cells by transient transfection of its cDNA, amplified by PCR, in pEF-BOS, also expressed in Escherichia coli system using pQE-32
Mus musculus
General Stability (protein specific)
EC Number
General Stability
Organism
1.13.11.40
leukotriene A synthase activity of the enzyme leveled off within 10 min, indicating suicide activation
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.40
AA861
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.40
2 arachidonic acid + 2 O2
Mus musculus
-
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.40
recombinant enzyme
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.40
skin
-
Mus musculus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.13.11.40
-70°C, crude and purified enzyme preparations can be stored without appreciable loss of activity for at least 6 months
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.40
2 arachidonic acid + 2 O2
-
439401
Mus musculus
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O
-
-
-
?
1.13.11.40
5-hydroperoxy fatty acid + O2
leukotriene A synthase activity, reaction rate is approximately 7% of arachidonate 8-lipoxygenation
439401
Mus musculus
leukotriene A4
-
-
-
?
1.13.11.40
5-hydroperoxyeicosatetraenoic acid + ?
-
439401
Mus musculus
leukotriene A4
unstable product
439401
Mus musculus
?
1.13.11.40
alpha-linolenic acid + O2
-
439401
Mus musculus
?
-
-
-
?
1.13.11.40
arachidonic acid + O2
-
439401
Mus musculus
(8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid
-
-
-
?
1.13.11.40
docosahexaenoic acid + O2
-
439401
Mus musculus
?
-
-
-
?
1.13.11.40
eicosapentaenoic acid + O2
-
439401
Mus musculus
?
-
-
-
?
1.13.11.40
gamma-linoleic acid + O2
-
439401
Mus musculus
?
-
-
-
?
1.13.11.40
linoleic acid + O2
poor substrate
439401
Mus musculus
?
-
-
-
?