EC Number | General Stability | Organism |
---|---|---|
1.13.11.5 | ascorbate stabilizes, it probably protects Fe2+ from spontaneous oxidation | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.5 | ascorbate | strong inhibitory effect, but stabilizes | Mus musculus | |
1.13.11.5 | Co2+ | incubation with Fe2+ plus Co2+ in equimolar concentrations inhibits | Mus musculus | |
1.13.11.5 | Cu2+ | incubation with Fe2+ plus Cu2+ in equimolar concentrations inhibits | Mus musculus | |
1.13.11.5 | glutathione | reduced glutathione slightly inhibits | Mus musculus | |
1.13.11.5 | additional information | not inhibited by Ca2+ and Mn2+ | Mus musculus | |
1.13.11.5 | Ni2+ | incubation with Fe2+ plus Ni2+ in equimolar concentrations inhibits | Mus musculus | |
1.13.11.5 | Zn2+ | incubation with Fe2+ plus Zn2+ in equimolar concentrations inhibits | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.5 | 0.188 | - |
homogentisate | - |
Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.13.11.5 | cytosol | - |
Mus musculus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.5 | Fe2+ | Fe2+ is an absolutely obligate cofactor and cannot be replaced by other divalent cations, highest enzyme activity in vitro at 2 mM Fe2+ | Mus musculus | |
1.13.11.5 | additional information | Fe3+ cannot replace Fe2+ | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.5 | 49000 | - |
3 * 49000, SDS-PAGE | Mus musculus |
1.13.11.5 | 149000 | - |
gel filtration | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.5 | homogentisate + O2 | Mus musculus | homogentisate is the natural substrate, key reaction in the catabolic pathway of aromatic amino acids, oxidative cleavage of the aromatic ring | 4-maleylacetoacetate | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.5 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.5 | 330fold purification | Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.5 | liver | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.5 | additional information | - |
- |
Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.5 | homogentisate + O2 | - |
Mus musculus | 4-maleylacetoacetate | - |
ir | |
1.13.11.5 | homogentisate + O2 | homogentisate is the natural substrate, key reaction in the catabolic pathway of aromatic amino acids, oxidative cleavage of the aromatic ring | Mus musculus | 4-maleylacetoacetate | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.5 | trimer | 3 * 49000, SDS-PAGE | Mus musculus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.5 | 25 | 55 | almost linear decrease of activity | Mus musculus |
1.13.11.5 | 45 | - |
65% loss of activity | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.5 | 6.1 | - |
- |
Mus musculus |