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Literature summary extracted from

  • Adachi, K.; Iwayama, Y.; Tanioka, H.; Takeda, Y.
    Purification and properties of homogentisate oxygenase from Pseudomonas fluorescens (1966), Biochim. Biophys. Acta, 118, 88-97.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.5 ascorbate required for maximal activity at pH 6 Pseudomonas fluorescens
1.13.11.5 cysteine activates Pseudomonas fluorescens
1.13.11.5 glutathione required for maximal activity at pH 6 and 5.4 Pseudomonas fluorescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.5
-
 Pseudomonas fluorescens

General Stability

EC Number General Stability Organism
1.13.11.5 unlike the mammalian enzyme, the bacterial enzyme is fairly stable on aging or during storage Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.5 2-mercaptoethanol
-
 Pseudomonas fluorescens
1.13.11.5 p-chloromercuribenzoate competitive inhibition with respect to Fe2+ but not to homogentisate Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.5 0.6
-
 homogentisate at pH 6 Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.5 Fe2+ Fe2+ is required as cofactor Pseudomonas fluorescens
1.13.11.5 additional information other metallic ions, such as Fe3+, Co2+, Mn2+, Cu+ and Cu2+, cannot replace Fe2+ Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.5 380000
-
 ultracentrifugal analysis Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.5 Pseudomonas fluorescens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.13.11.5 sensitive to O2, easily inactivated by aeration Pseudomonas fluorescens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.5
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.5 8.25
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.13.11.5 -20°C, pH 6.0-7.2, in absence of Fe2+, several weeks, stable Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.5 homogentisate + O2 specific for homogentisate Pseudomonas fluorescens 4-maleylacetoacetate
-
 ?
1.13.11.5 additional information not as substrates: salicylic acid, catechol, 2,3-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,6-dihydroxybenzoic acid, o-hydroxyphenylacetic acid, p-hydroxyphenylacetic acid, 3,4-dihydroxyphenylacetic acid Pseudomonas fluorescens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.5 30
-
 assay at Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.5 6
-
-
 Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.5 6 7.2 -20°C, stable for several weeks Pseudomonas fluorescens