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Literature summary extracted from
- Regulation of 3-indoleacetic acid production in Pseudomonas syringae pv. savastanoi. Purification and properties of tryptophan 2-monooxygenase (1985), J. Biol. Chem., 260, 6281-6287.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.13.12.3 | FAD has no effect on stability | Pseudomonas savastanoi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.3 | Indole-3-acetamide | end product inhibition | Pseudomonas savastanoi |  |
| 1.13.12.3 | indole-3-acetic acid | - |
Pseudomonas savastanoi | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.3 | 0.05 | - |
L-tryptophan | - |
Pseudomonas savastanoi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.3 | 62000 | - |
amino acid composition | Pseudomonas savastanoi |
| 1.13.12.3 | 85000 | - |
gel filtration | Pseudomonas savastanoi |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.3 | L-tryptophan + O2 | Pseudomonas savastanoi | - |
indole-3-acetamide + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.12.3 | Pseudomonas savastanoi | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.12.3 | - |
Pseudomonas savastanoi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.12.3 | 13.28 | - |
- |
Pseudomonas savastanoi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.12.3 | L-tryptophan + O2 | - |
Pseudomonas savastanoi | indole-3-acetamide + CO2 + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.12.3 | monomer | 1 * 62000, SDS-PAGE | Pseudomonas savastanoi |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.12.3 | FAD | enzyme contains 0.73 FAD molecules per subunit, addition of FAD to partially purified preparation has no effect on activity | Pseudomonas savastanoi | |
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