BRENDA - Enzyme Database show

Hydroxylation of gamma-butyrobetaine to carnitine in rat liver

Lindstedt, G.; Biochemistry 6, 1271-1282 (1967)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.11.1
catalase
-
Rattus norvegicus
1.14.11.1
Microsomal preparation
increase of activity
Rattus norvegicus
1.14.11.1
NADPH
NADPH-regenerating system, increase of activity, no absolute requirement
Rattus norvegicus
1.14.11.1
nicotinamide
increase of activity
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.1
ascorbate
-
Rattus norvegicus
1.14.11.1
FMN
in high concentrations
Rattus norvegicus
1.14.11.1
H2O2
-
Rattus norvegicus
1.14.11.1
Quinacrine
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.11.1
cytoplasm
-
Rattus norvegicus
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.1
Fe2+
requirement
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.11.1
Rattus norvegicus
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.14.11.1
O2, irreversible inactivation during preincubation
Rattus norvegicus
Purification (Commentary)
EC Number
Commentary
Organism
1.14.11.1
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.1
liver
-
Rattus norvegicus
-
1.14.11.1
additional information
no activity found: muscle, kidney
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.11.1
0.0014
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.1
3-trimethylaminopropionic acid + 2-oxoglutarate + O2
20% of the hydroxylation rate of gamma-butyrobetaine
439297
Rattus norvegicus
?
-
-
-
?
1.14.11.1
4-dimethylaminobutyric acid + 2-oxoglutarate + O2
poor substrate
439297
Rattus norvegicus
4-dimethylamino-3-hydroxybutyric acid + succinate + CO2
-
439297
Rattus norvegicus
?
1.14.11.1
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
439297
Rattus norvegicus
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
439297
Rattus norvegicus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.1
37
-
assay at
Rattus norvegicus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.11.1
60
-
complete inactivation after 15 min
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.11.1
6
8.4
half-maximal activity at pH 6.0 and 8.4, partially purified preparation
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.11.1
ascorbate
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.11.1
catalase
-
Rattus norvegicus
1.14.11.1
Microsomal preparation
increase of activity
Rattus norvegicus
1.14.11.1
NADPH
NADPH-regenerating system, increase of activity, no absolute requirement
Rattus norvegicus
1.14.11.1
nicotinamide
increase of activity
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.11.1
ascorbate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.11.1
ascorbate
-
Rattus norvegicus
1.14.11.1
FMN
in high concentrations
Rattus norvegicus
1.14.11.1
H2O2
-
Rattus norvegicus
1.14.11.1
Quinacrine
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.11.1
cytoplasm
-
Rattus norvegicus
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.1
Fe2+
requirement
Rattus norvegicus
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.14.11.1
O2, irreversible inactivation during preincubation
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.1
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.1
liver
-
Rattus norvegicus
-
1.14.11.1
additional information
no activity found: muscle, kidney
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.11.1
0.0014
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.1
3-trimethylaminopropionic acid + 2-oxoglutarate + O2
20% of the hydroxylation rate of gamma-butyrobetaine
439297
Rattus norvegicus
?
-
-
-
?
1.14.11.1
4-dimethylaminobutyric acid + 2-oxoglutarate + O2
poor substrate
439297
Rattus norvegicus
4-dimethylamino-3-hydroxybutyric acid + succinate + CO2
-
439297
Rattus norvegicus
?
1.14.11.1
4-trimethylammoniobutanoate + 2-oxoglutarate + O2
-
439297
Rattus norvegicus
3-hydroxy-4-trimethylammoniobutanoate + succinate + CO2
-
439297
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.11.1
37
-
assay at
Rattus norvegicus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.11.1
60
-
complete inactivation after 15 min
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.11.1
6
8.4
half-maximal activity at pH 6.0 and 8.4, partially purified preparation
Rattus norvegicus