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Literature summary extracted from

  • Hashimoto, T.; Yamada, Y.
    Hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, an alkaloid-producing root cultures (1986), Plant Physiol., 81, 619-625.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.11 acetone 10% v/v, activation Atropa belladonna
1.14.11.11 acetone 10% v/v, activation Duboisia leichhardtii
1.14.11.11 acetone 10% v/v, activation Hyoscyamus niger
1.14.11.11 acetone 10% v/v, activation Hyoscyamus muticus
1.14.11.11 acetone 10% v/v, activation Hyoscyamus pusillus
1.14.11.11 acetone 10% v/v, activation Hyoscyamus albus
1.14.11.11 acetone 10% v/v, activation Datura metel
1.14.11.11 acetone 10% v/v, activation Hyoscyamus gyorffi
1.14.11.11 ascorbate required Atropa belladonna
1.14.11.11 ascorbate required Duboisia leichhardtii
1.14.11.11 ascorbate required Hyoscyamus niger
1.14.11.11 ascorbate required Hyoscyamus muticus
1.14.11.11 ascorbate required Hyoscyamus pusillus
1.14.11.11 ascorbate required Hyoscyamus albus
1.14.11.11 ascorbate required Datura metel
1.14.11.11 ascorbate required Hyoscyamus gyorffi
1.14.11.11 catalase activation Atropa belladonna
1.14.11.11 catalase activation Duboisia leichhardtii
1.14.11.11 catalase activation Hyoscyamus niger
1.14.11.11 catalase activation Hyoscyamus muticus
1.14.11.11 catalase activation Hyoscyamus pusillus
1.14.11.11 catalase activation Hyoscyamus albus
1.14.11.11 catalase activation Datura metel
1.14.11.11 catalase activation Hyoscyamus gyorffi
1.14.11.11 dithiothreitol activation Atropa belladonna
1.14.11.11 dithiothreitol activation Duboisia leichhardtii
1.14.11.11 dithiothreitol activation Hyoscyamus niger
1.14.11.11 dithiothreitol activation Hyoscyamus muticus
1.14.11.11 dithiothreitol activation Hyoscyamus pusillus
1.14.11.11 dithiothreitol activation Hyoscyamus albus
1.14.11.11 dithiothreitol activation Datura metel
1.14.11.11 dithiothreitol activation Hyoscyamus gyorffi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.11 0.017
-
 L-hyoscyamine
-
 Hyoscyamus niger
1.14.11.11 0.057
-
 2-oxoglutarate
-
 Hyoscyamus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.11 Fe2+ required Atropa belladonna
1.14.11.11 Fe2+ required Duboisia leichhardtii
1.14.11.11 Fe2+ required Hyoscyamus niger
1.14.11.11 Fe2+ required Hyoscyamus muticus
1.14.11.11 Fe2+ required Hyoscyamus pusillus
1.14.11.11 Fe2+ required Hyoscyamus albus
1.14.11.11 Fe2+ required Datura metel
1.14.11.11 Fe2+ required Hyoscyamus gyorffi

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.11.11 Acetone 10% v/v, activation Atropa belladonna
1.14.11.11 Acetone 10% v/v, activation Duboisia leichhardtii
1.14.11.11 Acetone 10% v/v, activation Hyoscyamus niger
1.14.11.11 Acetone 10% v/v, activation Hyoscyamus muticus
1.14.11.11 Acetone 10% v/v, activation Hyoscyamus pusillus
1.14.11.11 Acetone 10% v/v, activation Hyoscyamus albus
1.14.11.11 Acetone 10% v/v, activation Datura metel
1.14.11.11 Acetone 10% v/v, activation Hyoscyamus gyorffi

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.11 Atropa belladonna
-
-
-
1.14.11.11 Datura metel
-
-
-
1.14.11.11 Duboisia leichhardtii
-
-
-
1.14.11.11 Hyoscyamus albus
-
-
-
1.14.11.11 Hyoscyamus gyorffi
-
-
-
1.14.11.11 Hyoscyamus muticus
-
-
-
1.14.11.11 Hyoscyamus niger
-
-
-
1.14.11.11 Hyoscyamus pusillus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.11 partial Hyoscyamus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.11 root culture Atropa belladonna
-
1.14.11.11 root culture Duboisia leichhardtii
-
1.14.11.11 root culture Hyoscyamus niger
-
1.14.11.11 root culture Hyoscyamus muticus
-
1.14.11.11 root culture Hyoscyamus pusillus
-
1.14.11.11 root culture Hyoscyamus albus
-
1.14.11.11 root culture Datura metel
-
1.14.11.11 root culture Hyoscyamus gyorffi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Atropa belladonna 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Duboisia leichhardtii 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus muticus 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus pusillus 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus albus 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Datura metel 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Hyoscyamus gyorffi 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2 only the L-isomer of hyoscyamine serves as substrate, D-hyoscyamine is nearly inactive Hyoscyamus niger 6beta-hydroxyhyoscyamine + succinate + CO2
-
 ?