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Literature summary extracted from

  • Griggs, D.L.; Hedden, P.; Lazarus, C.M.
    Partial purification of two gibberellin 2beta-hydroxylases from cotyledons of Phaseolus vulgaris (1991), Phytochemistry, 30, 2507-2512.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.13 0.000027
-
 [2,3-3H2]gibberellin 9 isoenzyme II Phaseolus vulgaris
1.14.11.13 0.000052
-
 [1,2-3H2]gibberellin 1 isoenzyme II Phaseolus vulgaris
1.14.11.13 0.00006
-
 [1,2-3H2]gibberellin 4 isoenzyme I Phaseolus vulgaris
1.14.11.13 0.000103
-
 [1,2-3H2]gibberellin 1 isoenzyme I Phaseolus vulgaris
1.14.11.13 0.000135
-
 [1,2-3H2]gibberellin 4 isoenzyme II Phaseolus vulgaris
1.14.11.13 0.000302
-
 [1,2,3-3H3]gibberellin 20 isoenzyme I Phaseolus vulgaris
1.14.11.13 0.000538
-
 [2,3-3H2]gibberellin 9 isoenzyme I Phaseolus vulgaris
1.14.11.13 0.0045
-
 [2,3-3H2]gibberellin 9 isoenzyme II Phaseolus vulgaris
1.14.11.13 0.0118
-
 [1,2-3H2]gibberellin 1 isoenzyme I Phaseolus vulgaris
1.14.11.13 0.01244
-
 [1,2,3-3H3]gibberellin 20 isoenzyme II Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.13 Fe2+
-
 Phaseolus vulgaris
1.14.11.13 Fe2+
-
 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.13 26000
-
 isoenzyme I, size exclusion column, SDS-PAGE Phaseolus vulgaris
1.14.11.13 36000
-
-
 Phaseolus vulgaris
1.14.11.13 42000
-
 isoenzyme II, gel filtration, SDS-PAGE Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.13 Phaseolus vulgaris
-
 var. Canadian Wonder
-
1.14.11.13 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.13 two gibberellin 2beta-hydroxylases partially purified Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.13 cotyledon
-
 Phaseolus vulgaris
-
1.14.11.13 cotyledon
-
 Pisum sativum
-
1.14.11.13 seed
-
 Pisum sativum
-
1.14.11.13 seed mature seeds Phaseolus vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.13 gibberellin 1 + 2-oxoglutarate + O2
-
 Phaseolus vulgaris 2beta-hydroxygibberellin 1 + succinate + CO2
-
 ?
1.14.11.13 gibberellin 1 + 2-oxoglutarate + O2
-
 Pisum sativum 2beta-hydroxygibberellin 1 + succinate + CO2
-
 ?
1.14.11.13 [1,2,3-3H3]gibberellin 20 + 2-oxoglutarate + O2
-
 Phaseolus vulgaris ?
-
 ?
1.14.11.13 [1,2-3H2]gibberellin 1 + 2-oxoglutarate + O2 isoenzyme I Phaseolus vulgaris ?
-
 ?
1.14.11.13 [2,3-3H2]gibberellin 9 + 2-oxoglutarate + O2 isoenzyme II Phaseolus vulgaris ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.13 5.8
-
-
 Phaseolus vulgaris
1.14.11.13 6 7 similar optimum for both isoenzymes Phaseolus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.13 2-oxoglutarate
-
 Phaseolus vulgaris
1.14.11.13 2-oxoglutarate
-
 Pisum sativum
1.14.11.13 ascorbate
-
 Phaseolus vulgaris
1.14.11.13 ascorbate
-
 Pisum sativum