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Literature summary extracted from

  • Smith, V.A.; MacMillan, J.
    The partial purification and characterisation of gibberellin 2beta-hydroxylases from seeds of Pisum sativum (1986), Planta, 167, 9-18.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.11.13 enzyme is very acid-labile. EDTA has beneficial effect on enzyme stability if Mg2+ is present in storage buffer Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.13 gibberellin 1 efficient inhibitor of gibberellin 20 2beta-hydroxylation Pisum sativum
1.14.11.13 Zn2+
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.13 0.000069
-
 gibberellin 1
-
 Pisum sativum
1.14.11.13 0.000299
-
 gibberellin 9
-
 Pisum sativum
1.14.11.13 0.00155
-
 gibberellin 20
-
 Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.13 Fe2+ activity dependent upon Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.13 43000
-
 major protein, SDS-PAGE Pisum sativum
1.14.11.13 44000
-
 gel filtration Pisum sativum
1.14.11.13 45000
-
 minor protein, SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.13 Pisum sativum
-
 garden pea, cv. progress No. 9
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.13
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.13 cotyledon
-
 Pisum sativum
-
1.14.11.13 embryo embryonic axes Pisum sativum
-
1.14.11.13 seed mature and immature seeds Pisum sativum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.13 gibberellin 1 + 2-oxoglutarate + O2
-
 Pisum sativum 2beta-hydroxygibberellin 1 + succinate + CO2
-
 ?
1.14.11.13 gibberellin 20 + 2-oxoglutarate + O2
-
 Pisum sativum gibberellin 29 + succinate + CO2
-
 ?
1.14.11.13 gibberellin 4 + 2-oxoglutarate + O2
-
 Pisum sativum gibberellin 34 + succinate + CO2
-
 ?
1.14.11.13 gibberellin 9 + 2-oxoglutarate + O2
-
 Pisum sativum gibberellin 51 + succinate + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.13 7.4 7.8
-
 Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.11.13 6.1 7.8
-
 Pisum sativum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.11.13 5.8 7.8 activity is highest at pH 7.0-8.0 and decreases rapidly below pH 7.0, enzyme is unstable when stored below pH 7 or in absence of a thiol reagent Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.13 2-oxoglutarate activity dependent upon Pisum sativum
1.14.11.13 ascorbate activity dependend upon Pisum sativum