Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • McGinnis, K.; Ku, G.M.; VanDusen, W.J.; Fu, J.; Garsky, V.; Stern, A.M.; Friedman, P.A.
    Site-directed mutagenesis of residues in a conserved region of bovine aspartyl (asparaginyl) beta-hydroxylase: Evidence that histidine 675 has a role in binding Fe2+ (1996), Biochemistry, 35, 3957-3962.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.16 wild-type and mutants expressed in Escherichia coli BL21 Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.16 G659A 21% activity of wild-type Bos taurus
1.14.11.16 G669A 90% activity of wild-type Bos taurus
1.14.11.16 H667L 106% activity of wild-type Bos taurus
1.14.11.16 H671L 16% activity of wild-type Bos taurus
1.14.11.16 H675D 20% activity of wild-type Bos taurus
1.14.11.16 H675E 12% activity of wild-type Bos taurus
1.14.11.16 H686L 9% activity of wild-type Bos taurus
1.14.11.16 P678V 90% activity of wild-type Bos taurus
1.14.11.16 R682A 10% activity of wild-type Bos taurus
1.14.11.16 R684A 8% activity of wild-type Bos taurus
1.14.11.16 R684K 87% activity of wild-type Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.16 0.013
-
 Fe2+ at 0.6 mM 2-oxoglutarate, wild-type Bos taurus
1.14.11.16 0.052
-
 Fe2+ at 0.6 mM 2-oxoglutarate, H675E mutant Bos taurus
1.14.11.16 0.093
-
 Fe2+ at 0.6 mM 2-oxoglutarate, H675D mutant Bos taurus
1.14.11.16 0.098
-
 2-oxoglutarate at 1 mM Fe2+, H675D mutant Bos taurus
1.14.11.16 0.102
-
 2-oxoglutarate at 1 mM Fe2+, wild-type Bos taurus
1.14.11.16 0.125
-
 2-oxoglutarate at 1 mM Fe2+, H675E mutant Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.16 Fe2+
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.16 Bos taurus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.16 peptide L-aspartate + 2-oxoglutarate + O2 second epidermal growth factor-like domain of bovine protein S Bos taurus peptide 3-hydroxy-L-aspartate + succinate + CO2
-
 ?