Literature summary extracted from
Veijola, J.; Koivunen, P.; Annunen, P.; Pihlajaniemi, T.; Kivirikko, K.I.
Cloning, baculovirus expression, and characterization of the a subunit of prolyl 4-hydroxylase from the nematode Caenorhabditis elegans. This a subunit forms an active ab dimer with the human protein disulfide isomerase/b subunit (1994), J. Biol. Chem., 269, 26746-26753.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.11.2 |
cloning of the alpha subunit of the enzyme, expression of the alpha subunit together with human protein disulfide isomerase/beta subunit in insect cells by baculovirus vectors |
Caenorhabditis elegans |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.11.2 |
poly(L-proline) |
not inhibitory |
Caenorhabditis elegans |
|
1.14.11.2 |
Pyridine 2,4-dicarboxylate |
recombinant enzyme, competitive inhibitor with respect to Fe2+ and 2-oxoglutarate |
Caenorhabditis elegans |
|
1.14.11.2 |
Zn2+ |
recombinant enzyme, competitive inhibition with respect to Fe2+ and 2-oxoglutarate |
Caenorhabditis elegans |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.14.11.2 |
0.015 |
- |
(L-Pro-L-Pro-Gly)10 |
recombinant enzyme |
Caenorhabditis elegans |
|
1.14.11.2 |
0.02 |
- |
2-oxoglutarate |
recombinant enzyme |
Caenorhabditis elegans |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.11.2 |
Fe2+ |
recombinant enzyme: Km 0.005 mM |
Caenorhabditis elegans |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.11.2 |
120000 |
- |
recombinant enzyme, gel filtration |
Caenorhabditis elegans |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.11.2 |
Caenorhabditis elegans |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.11.2 |
of the recombinant enzyme, using anion exchange chromatography on a DEAE-cellulose column and two gel filtration steps |
Caenorhabditis elegans |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.11.2 |
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2 |
- |
Caenorhabditis elegans |
(L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2 |
- |
r |
|
1.14.11.2 |
(Pro-Pro-Gly)n + 2-oxoglutarate + O2 |
n: 1,5,10 |
Caenorhabditis elegans |
(Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 |
n: 1,5,10 |
? |
|
1.14.11.2 |
poly(L-Pro) + 2-oxoglutarate + O2 |
do not serve as substrate, assayed with recombinant enzyme |
Caenorhabditis elegans |
poly(4-hydroxyproline) + succinate + CO2 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.11.2 |
dimer |
recombinant enzyme, alpha beta, ratio: 1 to 1 |
Caenorhabditis elegans |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.11.2 |
ascorbate |
recombinant enzyme, Km: 0.3 mM |
Caenorhabditis elegans |
|