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Literature summary extracted from

  • Kaska, D.D.; Myllylä, R.; Gunzler, V.; Gibori, A.; Kivirikko, K.I.
    Prolyl 4-hydroxylase from Volvox carteri. A low-Mr enzyme antigenically related to the alpha subunit of the vertebrate enzyme (1988), Biochem. J., 256, 257-263.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 3,4-Dihydroxyphenylacetate
-
 Helianthus tuberosus
1.14.11.2 3,4-Dihydroxyphenylacetate
-
 Persea americana
1.14.11.2 3,4-Dihydroxyphenylacetate
-
 Ulva intestinalis
1.14.11.2 3,4-Dihydroxyphenylacetate
-
 Volvox carteri
1.14.11.2 3,4-Dihydroxyphenylpropionate
-
 Helianthus tuberosus
1.14.11.2 3,4-Dihydroxyphenylpropionate
-
 Persea americana
1.14.11.2 3,4-Dihydroxyphenylpropionate
-
 Ulva intestinalis
1.14.11.2 3,4-Dihydroxyphenylpropionate
-
 Volvox carteri
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Volvox carteri
1.14.11.2 Pyridine 2,5-dicarboxylate
-
 Volvox carteri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.01
-
 poly(L-proline)
-
 Volvox carteri
1.14.11.2 0.17
-
 2-oxoglutarate
-
 Volvox carteri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Helianthus tuberosus
1.14.11.2 Fe2+
-
 Persea americana
1.14.11.2 Fe2+
-
 Ulva intestinalis
1.14.11.2 Fe2+ Km: 0.02 mM Volvox carteri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 40000
-
 gel filtration Ulva intestinalis
1.14.11.2 40000
-
 gel filtration Volvox carteri
1.14.11.2 65000
-
 1 * 65000, SDS-PAGE Volvox carteri

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Helianthus tuberosus
-
-
-
1.14.11.2 Persea americana
-
-
-
1.14.11.2 Ulva intestinalis
-
-
-
1.14.11.2 Volvox carteri
-
 green algae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 using ion-exchange chromatography on DEAE-cellulose, and affinity chromatography on poly(L-hydroxyproline), 3,4-dihydroxyphenylacetate or 3,4-dihydroxyphenylpropionate linked to Sepharose Volvox carteri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.2 additional information
-
-
 Helianthus tuberosus
1.14.11.2 additional information
-
-
 Persea americana
1.14.11.2 additional information
-
-
 Ulva intestinalis
1.14.11.2 additional information
-
-
 Volvox carteri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 additional information proline or the dipeptides Ser-Pro or Ala-Pro do not serve as substrates Volvox carteri ?
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2
-
 Helianthus tuberosus poly(4-hydroxyproline) + succinate + CO2
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2
-
 Persea americana poly(4-hydroxyproline) + succinate + CO2
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2
-
 Ulva intestinalis poly(4-hydroxyproline) + succinate + CO2
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2
-
 Volvox carteri poly(4-hydroxyproline) + succinate + CO2
-
 ?
1.14.11.2 poly(L-proline) + 2-oxoglutarate + O2
-
 Volvox carteri poly(trans-4-hydroxy-L-proline) + succinate + CO2
-
 r

Subunits

EC Number Subunits Comment Organism
1.14.11.2 monomer 1 * 65000, SDS-PAGE Volvox carteri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 40
-
-
 Volvox carteri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 6.6 6.8
-
 Volvox carteri

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate
-
 Helianthus tuberosus
1.14.11.2 ascorbate
-
 Persea americana
1.14.11.2 ascorbate
-
 Ulva intestinalis
1.14.11.2 ascorbate Km: 0.14 mM Volvox carteri

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.008
-
 Pyridine 2,4-dicarboxylate
-
 Volvox carteri
1.14.11.2 0.02
-
 Pyridine 2,5-dicarboxylate
-
 Volvox carteri
1.14.11.2 0.024
-
 3,4-Dihydroxyphenylpropionate
-
 Volvox carteri
1.14.11.2 0.04
-
 3,4-Dihydroxyphenylacetate
-
 Volvox carteri
1.14.11.2 3
-
 poly(L-hydroxyproline)
-
 Volvox carteri