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Literature summary extracted from

  • Blankenstein, P.; Lang, W.C.; Robinson, D.G.
    Isolation and characterization of prolyl hydroxylase from Chlamydomonas reinhardii (1986), Planta, 169, 238-244.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 NaCl at 0.5 mM Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.002
-
 poly(L-Pro) MW 7000 Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.2 microsome loosely associated to membrane Chlamydomonas reinhardtii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 60000
-
 2 * 60000 + 2 * 65000, SDS-PAGE Chlamydomonas reinhardtii
1.14.11.2 60000
-
 2 * 60000 + 2 * 65000 Chlamydomonas reinhardtii
1.14.11.2 65000
-
 2 * 60000 + 2 * 65000, SDS-PAGE Chlamydomonas reinhardtii
1.14.11.2 65000
-
 2 * 60000 + 2 * 65000 Chlamydomonas reinhardtii
1.14.11.2 250000
-
 native gel electrophoresis Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 partial, using ion-exchange chromatography on DEAE-cellulose, affinity chromatography with poly-L-proline coupled to tresyl-activated Sepharose-4B and gel filtration Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.2 0.000066
-
 purified enzyme, 6 M-urea-elution and dialysis Chlamydomonas reinhardtii
1.14.11.2 0.000786
-
 purified enzyme, poly-L-proline-eluted Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2
-
 ?
1.14.11.2 bradykinin + 2-oxoglutarate + O2
-
 Chlamydomonas reinhardtii 4-hydroxyproline containing bradykinin
-
 ?
1.14.11.2 poly(L-Pro) + 2-oxoglutarate + O2 MW 7000 Chlamydomonas reinhardtii poly(4-hydroxyproline) + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 tetramer 2 * 60000 + 2 * 65000, SDS-PAGE Chlamydomonas reinhardtii
1.14.11.2 tetramer 2 * 60000 + 2 * 65000 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 30
-
-
 Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 7
-
-
 Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 2-oxoglutarate
-
 Chlamydomonas reinhardtii