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Literature summary extracted from

  • Myllylä, R.; Tuderman, L.; Kivirikko, K.I.
    Mechanism of the prolyl hydroxylase reaction. 2. Kinetic analysis of the reaction sequence (1977), Eur. J. Biochem., 80, 349-357.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 dithiothreitol activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 CO2 5% inhibition at 3.6 mM, 35% inhibition at 7.2 mM and 75% inhibition at 12 mM Gallus gallus
1.14.11.2 Collagen product inhibitor, noncompetitive with respect to all substrates of the reaction Gallus gallus
1.14.11.2 poly(L-proline) competitive inhibitor with respect to the polypeptide substrate and uncompetitive with respect to Fe2+ and 2-oxoglutarate Gallus gallus
1.14.11.2 succinate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with respect to Fe2+ Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 additional information
-
 additional information
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.11.2 glycoprotein
-
 Gallus gallus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2 = procollagen trans-4-hydroxy-L-proline + succinate + CO2 mechanism, kinetic analysis of the reaction sequence Gallus gallus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate
-
 Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 1.4 1.6 succinate calculated from the slopes or intercepts Gallus gallus
1.14.11.2 2.3 2.5 poly(L-proline) calculated from the slopes or intercepts Gallus gallus
1.14.11.2 40
-
 Collagen from basement membranes, calculated from the slopes or intercepts Gallus gallus
1.14.11.2 170 180 Collagen from skin, calculated from the slopes or intercepts Gallus gallus