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Literature summary extracted from
- The kinetics of the hydroxylation of procollagen by prolyl 4-hydroxylase. Proposal for a processive mechanism of binding of the dimeric hydroxylating enzyme in relation to the high kcat/Km ratio and a conformational requirement for hydroxylation of -X-Pro-Gly- sequences (1991), Biochim. Biophys. Acta, 1079, 103-111.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.2 | bovine serum albumin | activation | Gallus gallus | |
| 1.14.11.2 | catalase | activation | Gallus gallus | |
| 1.14.11.2 | dithiothreitol | activation | Gallus gallus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.2 | 0.0000016 | - |
Procollagen L-proline | - |
Gallus gallus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.2 | Fe2+ | - |
Gallus gallus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.2 | Gallus gallus | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.11.2 | procollagen L-proline + 2-oxoglutarate + O2 = procollagen trans-4-hydroxy-L-proline + succinate + CO2 | mechanism | Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.11.2 | embryo | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.2 | procollagen L-proline + 2-oxoglutarate + O2 | - |
Gallus gallus | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.2 | 32 | - |
assay at | Gallus gallus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.2 | 4.8 | - |
Procollagen L-proline | - |
Gallus gallus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.2 | 7.4 | - |
- |
Gallus gallus |
| 1.14.11.2 | 7.7 | - |
assay at | Gallus gallus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.2 | ascorbate | - |
Gallus gallus | |
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Release 2023.1 (January 2023)
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