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Literature summary extracted from

  • Kivirikko, K.I.; Myllylä, R.
    Recent developments in posttranslational modification: intracellular processing (1987), Methods Enzymol., 144, 96-114.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 cysteine activation Gallus gallus
1.14.11.2 cysteine activation Homo sapiens
1.14.11.2 dithiothreitol activation Gallus gallus
1.14.11.2 dithiothreitol activation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.2 preparation of cDNA clones for the two subunits, expression in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 2,3-Dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 2,3-Dihydroxybenzoate
-
 Homo sapiens
1.14.11.2 3,4-dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 3,4-dihydroxybenzoate
-
 Homo sapiens
1.14.11.2 3,4-Dihydroxyphenylacetate
-
 Gallus gallus
1.14.11.2 3,4-Dihydroxyphenylacetate
-
 Homo sapiens
1.14.11.2 4-hydroxybenzoate
-
 Gallus gallus
1.14.11.2 4-hydroxybenzoate
-
 Homo sapiens
1.14.11.2 Cu2+
-
 Gallus gallus
1.14.11.2 Cu2+
-
 Homo sapiens
1.14.11.2 glutamyl-3,4-dehydroprolyl-bradykinin
-
 Gallus gallus
1.14.11.2 glutamyl-3,4-dehydroprolyl-bradykinin
-
 Homo sapiens
1.14.11.2 hydroxybenzene
-
 Gallus gallus
1.14.11.2 poly(L-proline)
-
 Gallus gallus
1.14.11.2 poly(L-proline)
-
 Homo sapiens
1.14.11.2 poly(L-proline)
-
 Mus musculus
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Gallus gallus
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Homo sapiens
1.14.11.2 Pyridine 2,4-dicarboxylate
-
 Mus musculus
1.14.11.2 Pyridine 2,5-dicarboxylate
-
 Gallus gallus
1.14.11.2 Pyridine 2,5-dicarboxylate
-
 Homo sapiens
1.14.11.2 pyridine 2-carboxylate
-
 Gallus gallus
1.14.11.2 Zn2+
-
 Gallus gallus
1.14.11.2 Zn2+
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+
-
 Gallus gallus
1.14.11.2 Fe2+
-
 Mus musculus
1.14.11.2 Fe2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.4.1 Proteins Homo sapiens native, reduced or with wrong disulfide bonds ?
-
 ?
5.3.4.1 Proteins Gallus gallus may play a role in retaining prolyl 4-hydroxylase in its native conformation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-
1.14.11.2 Homo sapiens
-
-
-
1.14.11.2 Mus musculus
-
-
-
5.3.4.1 Gallus gallus
-
-
-
5.3.4.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.11.2 glycoprotein carbohydrate composition Gallus gallus
1.14.11.2 glycoprotein carbohydrate composition Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.2 isolation of subunits Gallus gallus
1.14.11.2 using affinity chromatography, ion-exchange chromatography and gel filtration Gallus gallus
1.14.11.2 using affinity chromatography, ion-exchange chromatography and gel filtration Homo sapiens
5.3.4.1
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-
1.14.11.2 liver
-
 Gallus gallus
-
1.14.11.2 liver
-
 Mus musculus
-
1.14.11.2 liver
-
 Homo sapiens
-
1.14.11.2 placenta
-
 Homo sapiens
-
5.3.4.1 cartilage embryonal Gallus gallus
-
5.3.4.1 embryo
-
 Gallus gallus
-
5.3.4.1 fibroblast cultured, confluent Homo sapiens
-
5.3.4.1 fibroblast lung Homo sapiens
-
5.3.4.1 skin fibroblast cultured, confluent Homo sapiens
-
5.3.4.1 tendon embryonal Gallus gallus
-
5.3.4.1 WI-38 cell SV40 transformed, cultured, confluent, weak activity Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
5.3.4.1 -20°C, stable for several months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 proline containing peptide + 2-oxoglutarate + O2 the presence of 2-oxoglutarate is an absolute and highly specific requirement, the formation of 4-hydroxyproline is accompanied by a stoichiometric decarboxylation of 2-oxoglutarate, the oxygen of the hydroxyl group is derived from molecular oxygen, the other atom of the O2 molecule being incorporated into the succinate, the activated form of oxygen is probably superoxide Gallus gallus 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
1.14.11.2 proline containing peptide + 2-oxoglutarate + O2 the presence of 2-oxoglutarate is an absolute and highly specific requirement, the formation of 4-hydroxyproline is accompanied by a stoichiometric decarboxylation of 2-oxoglutarate, the oxygen of the hydroxyl group is derived from molecular oxygen, the other atom of the O2 molecule being incorporated into the succinate, the activated form of oxygen is probably superoxide Homo sapiens 4-hydroxyproline containing peptide + succinate + CO2
-
 ?
5.3.4.1 Proteins native, reduced or with wrong disulfide bonds Gallus gallus Proteins with correct disulfide bonds ?
5.3.4.1 Proteins native, reduced or with wrong disulfide bonds Homo sapiens Proteins with correct disulfide bonds ?
5.3.4.1 Proteins incorrectly disulfide-linked bovine pancreatic ribonuclease Gallus gallus Proteins with correct disulfide bonds ?
5.3.4.1 Proteins native, reduced or with wrong disulfide bonds Homo sapiens ?
-
 ?
5.3.4.1 Proteins may play a role in retaining prolyl 4-hydroxylase in its native conformation Gallus gallus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 tetramer fibroblasts, SDS-PAGE, due to a larger carbohydrate content the alpha-subunit also exists in larger forms Gallus gallus
1.14.11.2 tetramer newborn, SDS-PAGE, due to a larger carbohydrate content the alpha-subunit also exists in larger forms Mus musculus
1.14.11.2 tetramer liver, SDS-PAGE, due to a larger carbohydrate content the alpha-subunit also exists in larger forms Homo sapiens
1.14.11.2 tetramer alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE Gallus gallus
1.14.11.2 tetramer alpha2 beta2, alpha: 64000, beta: 60000, ratio 1 to 1, SDS-PAGE Homo sapiens
1.14.11.2 tetramer there are two forms of the alpha subunit Gallus gallus
1.14.11.2 tetramer there are two forms of the alpha subunit Mus musculus
1.14.11.2 tetramer there are two forms of the alpha subunit Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate
-
 Mus musculus
1.14.11.2 ascorbate highly specific for Gallus gallus
1.14.11.2 ascorbate highly specific for Homo sapiens
1.14.11.2 ascorbate oxygen acceptor in the decarboxylation of 2-oxoglutarate without subsequent hydroxylation of peptide substrate Gallus gallus
1.14.11.2 ascorbate oxygen acceptor in the decarboxylation of 2-oxoglutarate without subsequent hydroxylation of peptide substrate Homo sapiens